Phenotype of the Triplo-lethal locus of Drosophila melanogaster and its suppression by hyperoxia

Laura K. Smoyer, Douglas R. Dorer, Kenneth Nickerson, Alan C Christensen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The Triplo-lethal locus (Tpl) of Drosophila is both triplo-lethal and haploinsufficient, but the function of the locus is unknown. We have examined Tpl-aneuploid embryos and find that, in both trisomics and monosomics, the midgut shows extensive cell death and the tracheae are abnormal. Shortly thereafter, all tissues die. PCR-based genotyping of individual embryos and larvae show that this phenotype occurs in the trisomics after hatching and in the monosomics before hatching. Weak alleles of the interacting gene Su(Tpl) delay the death of Tpl trisomics, but they still show the same tracheal and midgut phenotypes before dying. Hyperoxia (45% oxygen) partially suppresses the phenotype of Tpl aneuploids, even though the use of a hypoxia reporter strain shows that dying Tpl aneuploids are not hypoxic. This is the first report of a phenotype associated with the Tpl locus and the first report of an environmental condition that suppresses the phenotype.

Original languageEnglish (US)
Pages (from-to)163-170
Number of pages8
JournalGenetical Research
Volume82
Issue number3
DOIs
StatePublished - Dec 1 2003

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Hyperoxia
Drosophila melanogaster
Phenotype
Aneuploidy
Embryonic Structures
Trachea
Drosophila
Larva
Cell Death
Alleles
Oxygen
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Phenotype of the Triplo-lethal locus of Drosophila melanogaster and its suppression by hyperoxia. / Smoyer, Laura K.; Dorer, Douglas R.; Nickerson, Kenneth; Christensen, Alan C.

In: Genetical Research, Vol. 82, No. 3, 01.12.2003, p. 163-170.

Research output: Contribution to journalArticle

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