Phenols removal by immobilized horseradish peroxidase

I. Alemzadeh, S. Nejati

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Application of immobilized horseradish peroxidase (HRP) in porous calcium alginate (ca-alginate) for the purpose of phenol removal is reported. The optimal conditions for immobilization of HRP in ca-alginate were identified. Gelation (encapsulation) was optimized at 1.0% (w/v) sodium alginate in the presence of 5.5% (w/v) of calcium chloride. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Increasing initial phenol concentration results in a decrease in % conversion. The highest conversion belongs to phenol concentration of 2 mM. Investigation into time course of phenol removal for both encapsulated and free enzymes showed that encapsulated enzyme had lower efficiency in comparison with the same concentration of free enzyme; however the capsules were reusable up to four cycles without any changes in their retention activity. Increasing enzyme concentration from 0.15 to 0.8 units/g alginate results in gradual increase in phenol removal. The ratio of hydrogen peroxide/phenol at which highest phenol removal obtained is found to be dependent on initial phenol concentration and in the solution of 2 and 8 mM phenol it was 1.15 and 0.94 respectively.

Original languageEnglish (US)
Pages (from-to)1082-1086
Number of pages5
JournalJournal of Hazardous Materials
Volume166
Issue number2-3
DOIs
StatePublished - Jul 30 2009

Fingerprint

Phenols
Horseradish Peroxidase
Phenol
phenol
alginate
Alginate
Enzymes
enzyme
calcium
Immobilization
immobilization
Calcium
removal
Sodium alginate
Calcium Chloride
Calcium chloride
encapsulation
Enzyme activity
Gelation
Encapsulation

Keywords

  • Calcium alginate
  • Encapsulation
  • Horseradish peroxidase (HRP)
  • Hydrogen peroxide
  • Phenol

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering

Cite this

Phenols removal by immobilized horseradish peroxidase. / Alemzadeh, I.; Nejati, S.

In: Journal of Hazardous Materials, Vol. 166, No. 2-3, 30.07.2009, p. 1082-1086.

Research output: Contribution to journalArticle

Alemzadeh, I. ; Nejati, S. / Phenols removal by immobilized horseradish peroxidase. In: Journal of Hazardous Materials. 2009 ; Vol. 166, No. 2-3. pp. 1082-1086.
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