Phenol sulfotransferase activities and localization in human nasal polyp epithelium

Joe D. Beckmann, Ron Bartzatt, Joseph Ulphani, Robert Palmatier, Joseph Harold Sisson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Nasal polyp epithelia, which exhibit a wide variation in epithelial cell morphologies, were tested for phenol sulfotransferase (PST) enzymes. Immunohistology revealed little or no detectable PST antigen in normal ciliated pseudostratified epithelia or in simple squamous metaplastic epithelia; however, intense expression was observed in regions of non-ciliated epithelial cell hyperplasia and in squamous epithelial cells overlying such hyperplastic sites. Western blots confirmed the presence of both P-PST (32 kDa) and M-PST (34 kDa) in the tissue extracts. Bimodal distribution of PST activity as a function of 4-nitrophenol concentration was consistent with expression of these two PST isoforms. These results indicate dynamic and epithelial differentiation-dependent expression of human PSTs in the nasal mucosa and suggest that these sulfotransferases can be modulated within human airways in vivo.

Original languageEnglish (US)
Pages (from-to)104-111
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume213
Issue number1
DOIs
StatePublished - Jan 1 1995

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Arylsulfotransferase
Nasal Polyps
Nasal Mucosa
Epithelial Cells
Epithelium
Sulfotransferases
Tissue Extracts
Hyperplasia
Protein Isoforms
Western Blotting
Antigens
Enzymes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Phenol sulfotransferase activities and localization in human nasal polyp epithelium. / Beckmann, Joe D.; Bartzatt, Ron; Ulphani, Joseph; Palmatier, Robert; Sisson, Joseph Harold.

In: Biochemical and Biophysical Research Communications, Vol. 213, No. 1, 01.01.1995, p. 104-111.

Research output: Contribution to journalArticle

Beckmann, Joe D. ; Bartzatt, Ron ; Ulphani, Joseph ; Palmatier, Robert ; Sisson, Joseph Harold. / Phenol sulfotransferase activities and localization in human nasal polyp epithelium. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 213, No. 1. pp. 104-111.
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