Pharmacological and genetic modulation of REV-ERB activity and expression affects orexigenic gene expression

Ariadna Amador, Yongjun Wang, Subhashis Banerjee, Theodore M. Kameneka, Laura A. Solt, Thomas P. Burris

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The nuclear receptors REV-ERBα and REV-ERBβ are transcription factors that play pivotal roles in the regulation of the circadian rhythm and various metabolic processes. The circadian rhythm is an endogenous mechanism, which generates entrainable biological changes that follow a 24-hour period. It regulates a number of physiological processes, including sleep/wakeful cycles and feeding behaviors. We recently demonstrated that REV-ERB-specific small molecules affect sleep and anxiety. The orexinergic system also plays a significant role in mammalian physiology and behavior, including the regulation of sleep and food intake. Importantly, orexin genes are expressed in a circadian manner. Given these overlaps in function and circadian expression, we wanted to determine whether the REV-ERBs might regulate orexin. We found that acute in vivo modulation of REV-ERB activity, with the REV-ERB-specific synthetic ligand SR9009, affects the circadian expression of orexinergic genes in mice. Long term dosing with SR9009 also suppresses orexinergic gene expression in mice. Finally, REV-ERBβ-deficient mice present with increased orexinergic transcripts. These data suggest that the REV-ERBs may be involved in the repression of orexinergic gene expression.

Original languageEnglish (US)
Article numbere0151014
JournalPloS one
Volume11
Issue number3
DOIs
StatePublished - Mar 2016

Fingerprint

sleep
Gene expression
Sleep
Modulation
Pharmacology
Circadian Rhythm
Gene Expression
gene expression
circadian rhythm
mice
Genes
Physiological Phenomena
Appetite Regulation
Physiology
Feeding Behavior
anxiety
Cytoplasmic and Nuclear Receptors
feeding behavior
food intake
Transcription Factors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Pharmacological and genetic modulation of REV-ERB activity and expression affects orexigenic gene expression. / Amador, Ariadna; Wang, Yongjun; Banerjee, Subhashis; Kameneka, Theodore M.; Solt, Laura A.; Burris, Thomas P.

In: PloS one, Vol. 11, No. 3, e0151014, 03.2016.

Research output: Contribution to journalArticle

Amador, Ariadna ; Wang, Yongjun ; Banerjee, Subhashis ; Kameneka, Theodore M. ; Solt, Laura A. ; Burris, Thomas P. / Pharmacological and genetic modulation of REV-ERB activity and expression affects orexigenic gene expression. In: PloS one. 2016 ; Vol. 11, No. 3.
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