Phagocyte-derived free radicals stimulated by ingestion of iron-rich Staphylococcus aureus: A spin-trapping study

Myron S. Cohen, Bradley E. Britigan, Yaachov S. Chai, Sovitj Pou, Thomas L. Roeder, Gerald M. Rosen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Phagocytic cells generate superoxide (O2-) and hydrogen peroxide (H2O2), creating the substrates for hydroxyl radical (HO.) in the presence of redox active metals. Previously it was shown that HO. is not a physiologic product of human neutrophils or monocytes but can be generated in the presence of high concentrations of iron. This study was undertaken to determine whether bacterial iron could be used for the generation of HO.. The growth of Staphylococcus aureus under iron-rich conditions increased bacterial iron concentration and phagocytosis of iron-rich bacteria allowed neutrophils to accumulate threefold more iron than ingestion of iron-starved organisms. Neither neutrophils nor monocytes ingesting iron-rich S. aureus generated iron-catalyzed HO. at levels detectable by spin-trapping techniques. No differences in the killing of iron-rich organisms by neutrophils was noted. The results suggest that HO. does not play a role in the killing of S. aureus by human neutrophils, regardless of their ability to deliver iron to the cell.

Original languageEnglish (US)
Pages (from-to)819-824
Number of pages6
JournalJournal of Infectious Diseases
Volume163
Issue number4
DOIs
StatePublished - Apr 1991

Fingerprint

Spin Trapping
Phagocytes
Free Radicals
Staphylococcus aureus
Iron
Eating
Neutrophils
Monocytes
Phagocytosis
Superoxides
Hydroxyl Radical
Hydrogen Peroxide
Oxidation-Reduction
Metals

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Phagocyte-derived free radicals stimulated by ingestion of iron-rich Staphylococcus aureus : A spin-trapping study. / Cohen, Myron S.; Britigan, Bradley E.; Chai, Yaachov S.; Pou, Sovitj; Roeder, Thomas L.; Rosen, Gerald M.

In: Journal of Infectious Diseases, Vol. 163, No. 4, 04.1991, p. 819-824.

Research output: Contribution to journalArticle

Cohen, Myron S. ; Britigan, Bradley E. ; Chai, Yaachov S. ; Pou, Sovitj ; Roeder, Thomas L. ; Rosen, Gerald M. / Phagocyte-derived free radicals stimulated by ingestion of iron-rich Staphylococcus aureus : A spin-trapping study. In: Journal of Infectious Diseases. 1991 ; Vol. 163, No. 4. pp. 819-824.
@article{fbbe8533ff7343b89cd0b399659c400c,
title = "Phagocyte-derived free radicals stimulated by ingestion of iron-rich Staphylococcus aureus: A spin-trapping study",
abstract = "Phagocytic cells generate superoxide (O2-) and hydrogen peroxide (H2O2), creating the substrates for hydroxyl radical (HO.) in the presence of redox active metals. Previously it was shown that HO. is not a physiologic product of human neutrophils or monocytes but can be generated in the presence of high concentrations of iron. This study was undertaken to determine whether bacterial iron could be used for the generation of HO.. The growth of Staphylococcus aureus under iron-rich conditions increased bacterial iron concentration and phagocytosis of iron-rich bacteria allowed neutrophils to accumulate threefold more iron than ingestion of iron-starved organisms. Neither neutrophils nor monocytes ingesting iron-rich S. aureus generated iron-catalyzed HO. at levels detectable by spin-trapping techniques. No differences in the killing of iron-rich organisms by neutrophils was noted. The results suggest that HO. does not play a role in the killing of S. aureus by human neutrophils, regardless of their ability to deliver iron to the cell.",
author = "Cohen, {Myron S.} and Britigan, {Bradley E.} and Chai, {Yaachov S.} and Sovitj Pou and Roeder, {Thomas L.} and Rosen, {Gerald M.}",
year = "1991",
month = "4",
doi = "10.1093/infdis/163.4.819",
language = "English (US)",
volume = "163",
pages = "819--824",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Phagocyte-derived free radicals stimulated by ingestion of iron-rich Staphylococcus aureus

T2 - A spin-trapping study

AU - Cohen, Myron S.

AU - Britigan, Bradley E.

AU - Chai, Yaachov S.

AU - Pou, Sovitj

AU - Roeder, Thomas L.

AU - Rosen, Gerald M.

PY - 1991/4

Y1 - 1991/4

N2 - Phagocytic cells generate superoxide (O2-) and hydrogen peroxide (H2O2), creating the substrates for hydroxyl radical (HO.) in the presence of redox active metals. Previously it was shown that HO. is not a physiologic product of human neutrophils or monocytes but can be generated in the presence of high concentrations of iron. This study was undertaken to determine whether bacterial iron could be used for the generation of HO.. The growth of Staphylococcus aureus under iron-rich conditions increased bacterial iron concentration and phagocytosis of iron-rich bacteria allowed neutrophils to accumulate threefold more iron than ingestion of iron-starved organisms. Neither neutrophils nor monocytes ingesting iron-rich S. aureus generated iron-catalyzed HO. at levels detectable by spin-trapping techniques. No differences in the killing of iron-rich organisms by neutrophils was noted. The results suggest that HO. does not play a role in the killing of S. aureus by human neutrophils, regardless of their ability to deliver iron to the cell.

AB - Phagocytic cells generate superoxide (O2-) and hydrogen peroxide (H2O2), creating the substrates for hydroxyl radical (HO.) in the presence of redox active metals. Previously it was shown that HO. is not a physiologic product of human neutrophils or monocytes but can be generated in the presence of high concentrations of iron. This study was undertaken to determine whether bacterial iron could be used for the generation of HO.. The growth of Staphylococcus aureus under iron-rich conditions increased bacterial iron concentration and phagocytosis of iron-rich bacteria allowed neutrophils to accumulate threefold more iron than ingestion of iron-starved organisms. Neither neutrophils nor monocytes ingesting iron-rich S. aureus generated iron-catalyzed HO. at levels detectable by spin-trapping techniques. No differences in the killing of iron-rich organisms by neutrophils was noted. The results suggest that HO. does not play a role in the killing of S. aureus by human neutrophils, regardless of their ability to deliver iron to the cell.

UR - http://www.scopus.com/inward/record.url?scp=0025729562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025729562&partnerID=8YFLogxK

U2 - 10.1093/infdis/163.4.819

DO - 10.1093/infdis/163.4.819

M3 - Article

C2 - 1849162

AN - SCOPUS:0025729562

VL - 163

SP - 819

EP - 824

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 4

ER -