Persistent expression of foreign genes in cultured hepatocytes

Expression vectors

Sonia M. Najjar, Robert E Lewis

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have optimized a liposome-based transfection method that mediated highly efficient stable expression of foreign genes in hepatocytes. Moreover, we have observed that the metallothionein 1 promoter in the bovine papilloma virus-based expression vector drove the highest expression of foreign genes in hepatocytes as compared with the cytomegalovirus and the human polypeptide chain elongation factor 1α (EF-1α) promoters in the pcDNA 3-based expression vector. The cytomegalovirus promoter failed to yield significant expression in these cells. Furthermore, expression of foreign genes persisted up to at least 15 passages when expression was under the control of either the EF-1α or the metallothionein 1 promoter. Thus, these two promoters led to comparable stability of foreign genes in hepatocytes, with the metallothionein 1 promoter yielding a higher level of expression of foreign genes in these cells.

Original languageEnglish (US)
Pages (from-to)41-45
Number of pages5
JournalGene
Volume230
Issue number1
DOIs
StatePublished - Apr 1 1999

Fingerprint

Metallothionein
Hepatocytes
Peptide Elongation Factor 1
Gene Expression
Cytomegalovirus
Papilloma
Liposomes
Transfection
Viruses
Peptides
Genes

Keywords

  • Bovine papilloma virus
  • Cytomegalovirus
  • Human polypeptide chain elongation factor 1α
  • Promoter
  • Stable transfection

ASJC Scopus subject areas

  • Genetics

Cite this

Persistent expression of foreign genes in cultured hepatocytes : Expression vectors. / Najjar, Sonia M.; Lewis, Robert E.

In: Gene, Vol. 230, No. 1, 01.04.1999, p. 41-45.

Research output: Contribution to journalArticle

@article{d5f180296b304b7e8b3d6bad0eac35df,
title = "Persistent expression of foreign genes in cultured hepatocytes: Expression vectors",
abstract = "We have optimized a liposome-based transfection method that mediated highly efficient stable expression of foreign genes in hepatocytes. Moreover, we have observed that the metallothionein 1 promoter in the bovine papilloma virus-based expression vector drove the highest expression of foreign genes in hepatocytes as compared with the cytomegalovirus and the human polypeptide chain elongation factor 1α (EF-1α) promoters in the pcDNA 3-based expression vector. The cytomegalovirus promoter failed to yield significant expression in these cells. Furthermore, expression of foreign genes persisted up to at least 15 passages when expression was under the control of either the EF-1α or the metallothionein 1 promoter. Thus, these two promoters led to comparable stability of foreign genes in hepatocytes, with the metallothionein 1 promoter yielding a higher level of expression of foreign genes in these cells.",
keywords = "Bovine papilloma virus, Cytomegalovirus, Human polypeptide chain elongation factor 1α, Promoter, Stable transfection",
author = "Najjar, {Sonia M.} and Lewis, {Robert E}",
year = "1999",
month = "4",
day = "1",
doi = "10.1016/S0378-1119(99)00052-9",
language = "English (US)",
volume = "230",
pages = "41--45",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Persistent expression of foreign genes in cultured hepatocytes

T2 - Expression vectors

AU - Najjar, Sonia M.

AU - Lewis, Robert E

PY - 1999/4/1

Y1 - 1999/4/1

N2 - We have optimized a liposome-based transfection method that mediated highly efficient stable expression of foreign genes in hepatocytes. Moreover, we have observed that the metallothionein 1 promoter in the bovine papilloma virus-based expression vector drove the highest expression of foreign genes in hepatocytes as compared with the cytomegalovirus and the human polypeptide chain elongation factor 1α (EF-1α) promoters in the pcDNA 3-based expression vector. The cytomegalovirus promoter failed to yield significant expression in these cells. Furthermore, expression of foreign genes persisted up to at least 15 passages when expression was under the control of either the EF-1α or the metallothionein 1 promoter. Thus, these two promoters led to comparable stability of foreign genes in hepatocytes, with the metallothionein 1 promoter yielding a higher level of expression of foreign genes in these cells.

AB - We have optimized a liposome-based transfection method that mediated highly efficient stable expression of foreign genes in hepatocytes. Moreover, we have observed that the metallothionein 1 promoter in the bovine papilloma virus-based expression vector drove the highest expression of foreign genes in hepatocytes as compared with the cytomegalovirus and the human polypeptide chain elongation factor 1α (EF-1α) promoters in the pcDNA 3-based expression vector. The cytomegalovirus promoter failed to yield significant expression in these cells. Furthermore, expression of foreign genes persisted up to at least 15 passages when expression was under the control of either the EF-1α or the metallothionein 1 promoter. Thus, these two promoters led to comparable stability of foreign genes in hepatocytes, with the metallothionein 1 promoter yielding a higher level of expression of foreign genes in these cells.

KW - Bovine papilloma virus

KW - Cytomegalovirus

KW - Human polypeptide chain elongation factor 1α

KW - Promoter

KW - Stable transfection

UR - http://www.scopus.com/inward/record.url?scp=0033119849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033119849&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(99)00052-9

DO - 10.1016/S0378-1119(99)00052-9

M3 - Article

VL - 230

SP - 41

EP - 45

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -