Persistence of human immunodeficiency virus (HIV) type 1 DNA in peripheral blood despite prolonged suppression of plasma HIV-1 RNA in children

Akihiko Saitoh, Karen Hsia, Terence Fenton, Christine A. Powell, Cindy Christopherson, Courtney V. Fletcher, Stuart E. Starr, Stephen A. Spector

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Abstract

Human immunodeficiency virus (HIV) type 1 DNA in peripheral blood mononuclear cells (PBMC) was quantified in 31 children who received efavirenz, nelfinavir, and 1 or 2 nucleoside reverse-transcriptase inhibitors for ≥2 years and in whom undetectable plasma HIV-1 RNA levels (<50 copies/mL) were sustained, to determine the usefulness of HIV-1 DNA as a marker of virus suppression. The median baseline HIV-1 DNA level was 750 copies/106PBMC. After initiation of highly active antiretroviral therapy (HAART), HIV-1 DNA levels decreased gradually, reaching a plateau from week 80 through week 104 (median HIV-1 DNA level, 263 copies/106PBMC). Children who had plasma HIV-1 RNA levels <50 copies/mL after receiving HAART for 8 weeks (n = 16) had persistently lower quantities of intracellular HIV-1 DNA than children whose HIV-1 RNA levels reached <50 copies/mL after 8 weeks of HAART (n = 15). The median half-life for intracellular HIV-1 DNA was 60 weeks. Thus, despite prolonged maintenance of undetectable levels of plasma HIV-1 RNA, HIV-1 DNA remains detectable in PBMC of children and may be a useful marker of further virus suppression.

Original languageEnglish (US)
Pages (from-to)1409-1416
Number of pages8
JournalJournal of Infectious Diseases
Volume185
Issue number10
DOIs
StatePublished - May 15 2002

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HIV-1
RNA
DNA
Highly Active Antiretroviral Therapy
efavirenz
Blood Cells
Nelfinavir
Viruses
Reverse Transcriptase Inhibitors
Nucleosides
Half-Life
Maintenance

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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Persistence of human immunodeficiency virus (HIV) type 1 DNA in peripheral blood despite prolonged suppression of plasma HIV-1 RNA in children. / Saitoh, Akihiko; Hsia, Karen; Fenton, Terence; Powell, Christine A.; Christopherson, Cindy; Fletcher, Courtney V.; Starr, Stuart E.; Spector, Stephen A.

In: Journal of Infectious Diseases, Vol. 185, No. 10, 15.05.2002, p. 1409-1416.

Research output: Contribution to journalArticle

Saitoh, Akihiko ; Hsia, Karen ; Fenton, Terence ; Powell, Christine A. ; Christopherson, Cindy ; Fletcher, Courtney V. ; Starr, Stuart E. ; Spector, Stephen A. / Persistence of human immunodeficiency virus (HIV) type 1 DNA in peripheral blood despite prolonged suppression of plasma HIV-1 RNA in children. In: Journal of Infectious Diseases. 2002 ; Vol. 185, No. 10. pp. 1409-1416.
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