Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance

Laurence V. Bindschedler, Julia Dewdney, Kris A. Blee, Julie M Stone, Tsuneaki Asai, Julia Plotnikov, Carine Denoux, Tezni Hayes, Chris Gerrish, Dewi R. Davies, Frederick M. Ausubel, G. Paul Bolwell

Research output: Contribution to journalArticle

317 Citations (Scopus)

Abstract

The oxidative burst is an early response to pathogen attack leading to the production of reactive oxygen species (ROS) including hydrogen peroxide. Two major mechanisms involving either NADPH oxidases or peroxidases that may exist singly or in combination in different plant species have been proposed for the generation of ROS. We identified an Arabidopsis thaliana azide-sensitive but diphenylene iodonium-insensitive apoplastic oxidative burst that generates H2O2 in response to a Fusarium oxysporum cell-wall preparation. Transgenic Arabidopsis plants expressing an anti-sense cDNA encoding a type III peroxidase, French bean peroxidase type 1 (FBP1) exhibited an impaired oxidative burst and were more susceptible than wild-type plants to both fungal and bacterial pathogens. Transcriptional profiling and RT-PCR analysis showed that the anti-sense (FBP1) transgenic plants had reduced levels of specific peroxidase-encoding mRNAs, including mRNAs corresponding to Arabidopsis genes At3g49120 (AtPCb) and At3g49110 (AtPCa) that encode two class III peroxidases with a high degree of homology to FBP1. These data indicate that peroxidases play a significant role in generating H2O2 during the Arabidopsis defense response and in conferring resistance to a wide range of pathogens.

Original languageEnglish (US)
Pages (from-to)851-863
Number of pages13
JournalPlant Journal
Volume47
Issue number6
DOIs
StatePublished - Sep 1 2006

Fingerprint

Respiratory Burst
Arabidopsis
Peroxidase
peroxidase
peroxidases
green beans
pathogens
Peroxidases
Genetically Modified Plants
reactive oxygen species
Reactive Oxygen Species
azides
Messenger RNA
Azides
NADPH Oxidase
Fusarium
Fusarium oxysporum
Cell Wall
Hydrogen Peroxide
hydrogen peroxide

Keywords

  • Apoplastic peroxidase
  • Arabidopsis
  • Oxidative burst
  • Reactive oxygen species

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Bindschedler, L. V., Dewdney, J., Blee, K. A., Stone, J. M., Asai, T., Plotnikov, J., ... Bolwell, G. P. (2006). Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance. Plant Journal, 47(6), 851-863. https://doi.org/10.1111/j.1365-313X.2006.02837.x

Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance. / Bindschedler, Laurence V.; Dewdney, Julia; Blee, Kris A.; Stone, Julie M; Asai, Tsuneaki; Plotnikov, Julia; Denoux, Carine; Hayes, Tezni; Gerrish, Chris; Davies, Dewi R.; Ausubel, Frederick M.; Bolwell, G. Paul.

In: Plant Journal, Vol. 47, No. 6, 01.09.2006, p. 851-863.

Research output: Contribution to journalArticle

Bindschedler, LV, Dewdney, J, Blee, KA, Stone, JM, Asai, T, Plotnikov, J, Denoux, C, Hayes, T, Gerrish, C, Davies, DR, Ausubel, FM & Bolwell, GP 2006, 'Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance', Plant Journal, vol. 47, no. 6, pp. 851-863. https://doi.org/10.1111/j.1365-313X.2006.02837.x
Bindschedler, Laurence V. ; Dewdney, Julia ; Blee, Kris A. ; Stone, Julie M ; Asai, Tsuneaki ; Plotnikov, Julia ; Denoux, Carine ; Hayes, Tezni ; Gerrish, Chris ; Davies, Dewi R. ; Ausubel, Frederick M. ; Bolwell, G. Paul. / Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance. In: Plant Journal. 2006 ; Vol. 47, No. 6. pp. 851-863.
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