Peritubular capillary dysfunction and renal tubular epithelial cell stress following lipopolysaccharide administration in mice

Liping Wu, Manish M. Tiwari, Kurt J. Messer, Joseph H. Holthoff, Neriman Gokden, Robert W. Brock, Philip R. Mayeux

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The mortality rate for septic patients with acute renal failure is extremely high. Since sepsis is often caused by lipopolysaccharide (LPS), a model of LPS challenge was used to study the development of kidney injury. Intravital video microscopy was utilized to investigate renal peritubular capillary blood flow in anesthetized male C57BL/6 mice at 0, 2, 6, 10, 18, 24, 36, and 48 h after LPS administration (10 mg/kg ip). As early as 2 h, capillary perfusion was dramatically compromised. Vessels with continuous flow were decreased from 89 ± 4% in saline controls to 57 ± 5% in LPS-treated mice (P < 0.01), and vessels with intermittent flow were increased from 6 ± 2% to 31 ± 5% (P < 0.01). At 2 h, mRNA for intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 were elevated 50- and 27-fold, respectively, suggesting that vascular inflammation is an early event that may contribute to capillary dysfunction. By 10 h, vessels with no flow increased from 5 ± 2% in saline controls to 19 ± 3% in LPS-treated mice (P < 0.05). By 48 h, capillary function was returning toward control levels. The decline in functional capillaries preceded the development of renal failure and was paralleled by induction of inducible nitric oxide synthase in the kidney. Using NAD(P)H autofluorescence as an indicator of cellular redox stress, we found that tubular cell stress was highly correlated with the percentage of dysfunctional capillaries (r2 = 0.8951, P < 0.0001). These data show that peritubular capillary dysfunction is an early event that contributes to tubular stress and renal injury.

Original languageEnglish (US)
Pages (from-to)F261-F268
JournalAmerican Journal of Physiology - Renal Physiology
Volume292
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Lipopolysaccharides
Epithelial Cells
Kidney
Video Microscopy
Vascular Cell Adhesion Molecule-1
Wounds and Injuries
Nitric Oxide Synthase Type II
Intercellular Adhesion Molecule-1
Inbred C57BL Mouse
Acute Kidney Injury
NAD
Oxidation-Reduction
Renal Insufficiency
Blood Vessels
Sepsis
Perfusion
Inflammation
Messenger RNA
Mortality

Keywords

  • Inducible nitric oxide synthase
  • Intercellular adhesion molecule-1
  • Intravital video microscopy
  • Sepsis
  • Vascular cell adhesion molecule-1

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Peritubular capillary dysfunction and renal tubular epithelial cell stress following lipopolysaccharide administration in mice. / Wu, Liping; Tiwari, Manish M.; Messer, Kurt J.; Holthoff, Joseph H.; Gokden, Neriman; Brock, Robert W.; Mayeux, Philip R.

In: American Journal of Physiology - Renal Physiology, Vol. 292, No. 1, 01.01.2007, p. F261-F268.

Research output: Contribution to journalArticle

Wu, Liping ; Tiwari, Manish M. ; Messer, Kurt J. ; Holthoff, Joseph H. ; Gokden, Neriman ; Brock, Robert W. ; Mayeux, Philip R. / Peritubular capillary dysfunction and renal tubular epithelial cell stress following lipopolysaccharide administration in mice. In: American Journal of Physiology - Renal Physiology. 2007 ; Vol. 292, No. 1. pp. F261-F268.
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