PEG-mediated synthesis of highly dispersive multifunctional superparamagnetic nanoparticles: Their physicochemical properties and function in vivo

Conroy Sun, Kim Du, Chen Fang, Narayan Bhattarai, Omid Veiseh, Forrest Kievit, Zachary Stephen, Donghoon Lee, Richard G. Ellenbogen, Buddy Ratner, Miqin Zhang

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Multifunctional superparamagnetic nanoparticles have been developed for a wide range of applications in nanomedicine, such as serving as tumor-targeted drug carriers and molecular imaging agents. To function in vivo, the development of these novel materials must overcome several challenging requirements including biocompatibility, stability in physiological solutions, nontoxicity, and the ability to traverse biological barriers. Here we report a PEG-mediated synthesis process to produce well-dispersed, ultrafine, and highly stable iron oxide nanoparticles for in vivo applications. Utilizing a biocompatible PEG coating bearing amine functional groups, the produced nanoparticles serve as an effective platform with the ability to incorporate a variety of targeting, therapeutic, or imaging ligands. In this study, we demonstrated tumor-specific accumulation of these nanoparticles through both magnetic resonance and optical imaging after conjugation with chlorotoxin, a peptide with high affinity toward tumors of the neuroectodermal origin, and Cy5.5, a near-infrared fluorescent dye. Furthermore, we performed preliminary biodistribution and toxicity assessments of these nanoparticles in wild-type mice through histological analysis of clearance organs and hematology assay, and the results demonstrated the relative biocompatibility of these nanoparticles.

Original languageEnglish (US)
Pages (from-to)2402-2410
Number of pages9
JournalACS Nano
Volume4
Issue number4
DOIs
StatePublished - Apr 27 2010

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Polyethylene glycols
Nanoparticles
nanoparticles
synthesis
Tumors
tumors
biocompatibility
Biocompatibility
hematology
Bearings (structural)
Medical nanotechnology
Molecular imaging
Imaging techniques
Drug Carriers
clearances
Magnetic resonance
conjugation
Iron oxides
Fluorescent Dyes
iron oxides

Keywords

  • Cancer
  • Chlorotoxin
  • Iron oxide nanoparticle
  • MRI
  • Nanomedicine
  • Optical imaging
  • PEG
  • Targeting

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

PEG-mediated synthesis of highly dispersive multifunctional superparamagnetic nanoparticles : Their physicochemical properties and function in vivo. / Sun, Conroy; Du, Kim; Fang, Chen; Bhattarai, Narayan; Veiseh, Omid; Kievit, Forrest; Stephen, Zachary; Lee, Donghoon; Ellenbogen, Richard G.; Ratner, Buddy; Zhang, Miqin.

In: ACS Nano, Vol. 4, No. 4, 27.04.2010, p. 2402-2410.

Research output: Contribution to journalArticle

Sun, C, Du, K, Fang, C, Bhattarai, N, Veiseh, O, Kievit, F, Stephen, Z, Lee, D, Ellenbogen, RG, Ratner, B & Zhang, M 2010, 'PEG-mediated synthesis of highly dispersive multifunctional superparamagnetic nanoparticles: Their physicochemical properties and function in vivo', ACS Nano, vol. 4, no. 4, pp. 2402-2410. https://doi.org/10.1021/nn100190v
Sun, Conroy ; Du, Kim ; Fang, Chen ; Bhattarai, Narayan ; Veiseh, Omid ; Kievit, Forrest ; Stephen, Zachary ; Lee, Donghoon ; Ellenbogen, Richard G. ; Ratner, Buddy ; Zhang, Miqin. / PEG-mediated synthesis of highly dispersive multifunctional superparamagnetic nanoparticles : Their physicochemical properties and function in vivo. In: ACS Nano. 2010 ; Vol. 4, No. 4. pp. 2402-2410.
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