PDGF-BB induced chemotaxis is impaired in aged capillary endothelial cells

Gregg D. Phillips, Anna Marika Stone

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The object of this study was to determine if the decreased angiogenesis in the healing wounds of the aged is due to the failure of endothelial cells to respond to locally produced growth factors. Endothelial cells isolated from wound sponges implanted in aged (24-month-old) and young (2-month-old) rats were tested for their chemotactic response to the BB isoform of platelet-derived growth factor (PDGF-BB). A similar number of cells isolated from both old and young rats stained positive (75-90%) for acetylated-LDL uptake, and the same number of viable cells was used in the chemotaxis assay. Endothelial cells from both old and young rats migrated in a dose-dependent (0.1-3.0 ng/ml) manner in response to PDGF-BB. At all concentrations tested, PDGF-BB elicited the migration of more endothelial cells from the young rats. The difference between the number of young and old cells that responded to PDGF-BB was statistically significant at the 1.0 ng/ml and 3.0 ng/ml concentrations. These results suggest that the impaired angiogenic response in the healing of wounds of the aged is due to altered endothelial cell reaction to the growth factors in the wound microenvironment.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalMechanisms of Ageing and Development
Volume73
Issue number3
DOIs
StatePublished - Jan 1 1994

Fingerprint

Endothelial cells
Chemotaxis
Endothelial Cells
Rats
Wound Healing
Intercellular Signaling Peptides and Proteins
Cell Count
Platelet-Derived Growth Factor
Wounds and Injuries
Porifera
Assays
Protein Isoforms
platelet-derived growth factor BB

Keywords

  • Aging
  • Angiogenesis
  • Endothelial cells
  • PDGF

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

Cite this

PDGF-BB induced chemotaxis is impaired in aged capillary endothelial cells. / Phillips, Gregg D.; Stone, Anna Marika.

In: Mechanisms of Ageing and Development, Vol. 73, No. 3, 01.01.1994, p. 189-196.

Research output: Contribution to journalArticle

Phillips, Gregg D. ; Stone, Anna Marika. / PDGF-BB induced chemotaxis is impaired in aged capillary endothelial cells. In: Mechanisms of Ageing and Development. 1994 ; Vol. 73, No. 3. pp. 189-196.
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