PD-1 and CTLA-4 up regulation on donor T cells is insufficient to prevent GvHD in allo-HSCT recipients

Mohammad S. Hossain, Ghada M. Kunter, Vicky F. El-Najjar, David L. Jaye, Zaid Al-Kadhimi, Owonikoko K. Taofeek, Jian Ming Li, Edmund K. Waller

Research output: Contribution to journalArticle

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Abstract

The expression of checkpoint blockade molecules PD-1, PD-L1, CTLA-4, and foxp3 +CD25+CD4+ T cells (Tregs) regulate donor T cell activation and graft-vs-host disease (GvHD) in allogeneic hematopoietic stem cell transplant (allo-HSCT). Detailed kinetics of PD-1-, CTLA-4-, and PD-L1 expression on donor and host cells in GvHD target organs have not been well studied. Using an established GvHD model of allo-HSCT (B6 ! CB6F1), we noted transient increases of PD-1- and CTLA-4-expressing donor CD4+ and CD8+ T cells on day 10 post transplant in spleens of allo-HSCT recipients compared with syngeneic HSCT (syn-HSCT) recipients. In contrast, expression of PD-1- and CTLA-4 on donor T cells was persistently increased in bone marrow (BM) of allo-HSCT recipients compared with syn-HSCT recipients. Similar differential patterns of donor T cell immune response were observed in a minor histocompatibility (miHA) mismatched transplant model of GvHD. Despite higher PD-1 and CTLA-4 expression in BM, numbers of foxp3+ T cells and Tregs were much lower in allo-HSCT recipients compared with syn-HSCT recipients. PD-L1-expressing host cells were markedly decreased concomitant with elimination of residual host hematopoietic elements in spleens of allo-HSCT recipients. Allo-HSCT recipients lacking PD-L1 rapidly developed increased serum inflammatory cytokines and lethal acute GvHD compared with wild-type (WT) B6 allo-HSCT recipients. These data suggest that increased expression of checkpoint blockade molecules PD-1 and CTLA-4 on donor T cells is not sufficient to prevent GvHD, and that cooperation between checkpoint blockade signaling by host cells and donor Tregs is necessary to limit GvHD in allo-HSCT recipients.

Original languageEnglish (US)
Article numbere0184254
JournalPloS one
Volume12
Issue number9
DOIs
StatePublished - Sep 2017

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Transplants
T-cells
Graft vs Host Disease
Hematopoietic Stem Cells
Stem cells
Grafts
Up-Regulation
T-lymphocytes
Tissue Donors
T-Lymphocytes
bone marrow
spleen
Bone
Spleen
Bone Marrow
Transplant Recipients
hematopoietic stem cells
disease models
cells
Molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hossain, M. S., Kunter, G. M., El-Najjar, V. F., Jaye, D. L., Al-Kadhimi, Z., Taofeek, O. K., ... Waller, E. K. (2017). PD-1 and CTLA-4 up regulation on donor T cells is insufficient to prevent GvHD in allo-HSCT recipients. PloS one, 12(9), [e0184254]. https://doi.org/10.1371/journal.pone.0184254

PD-1 and CTLA-4 up regulation on donor T cells is insufficient to prevent GvHD in allo-HSCT recipients. / Hossain, Mohammad S.; Kunter, Ghada M.; El-Najjar, Vicky F.; Jaye, David L.; Al-Kadhimi, Zaid; Taofeek, Owonikoko K.; Li, Jian Ming; Waller, Edmund K.

In: PloS one, Vol. 12, No. 9, e0184254, 09.2017.

Research output: Contribution to journalArticle

Hossain, MS, Kunter, GM, El-Najjar, VF, Jaye, DL, Al-Kadhimi, Z, Taofeek, OK, Li, JM & Waller, EK 2017, 'PD-1 and CTLA-4 up regulation on donor T cells is insufficient to prevent GvHD in allo-HSCT recipients', PloS one, vol. 12, no. 9, e0184254. https://doi.org/10.1371/journal.pone.0184254
Hossain, Mohammad S. ; Kunter, Ghada M. ; El-Najjar, Vicky F. ; Jaye, David L. ; Al-Kadhimi, Zaid ; Taofeek, Owonikoko K. ; Li, Jian Ming ; Waller, Edmund K. / PD-1 and CTLA-4 up regulation on donor T cells is insufficient to prevent GvHD in allo-HSCT recipients. In: PloS one. 2017 ; Vol. 12, No. 9.
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AU - Jaye, David L.

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