PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis

He Huang, Chan Yul Yoo, Rebecca Bindbeutel, Jessica Goldsworthy, Allison Tielking, Sophie Alvarez, Michael J. Naldrett, Bradley S. Evans, Meng Chen, Dmitri A. Nusinow

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Plants react to seasonal change in day length through altering physiology and development. Factors that function to harmonize growth with photoperiod are poorly understood. Here we characterize a new protein that associates with both circadian clock and photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens hypocotyls independent of day length. PCH1 peaks at dusk, binds phytochrome B (phyB) in a red light-dependent manner, and co-localizes with phyB into photobodies. PCH1 is necessary and sufficient to promote the biogenesis of large photobodies to maintain an active phyB pool after light exposure, potentiating red-light signaling and prolonging memory of prior illumination. Manipulating PCH1 alters PHYTOCHROME INTERACTING FACTOR 4 levels and regulates light-responsive gene expression. Thus, PCH1 is a new factor that regulates photoperiod-responsive growth by integrating the clock with light perception pathways through modulating daily phyB-signaling.

Original languageEnglish (US)
Article numbere13292
JournaleLife
Volume5
Issue numberFEBRUARY2016
DOIs
StatePublished - Feb 3 2016

Fingerprint

Phytochrome B
Photoperiod
Arabidopsis
Light
Growth
Hypocotyl
Clocks
Phytochrome
Circadian Clocks
Lighting
Seedlings
Physiology
Gene expression
Gene Expression
Data storage equipment
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Huang, H., Yoo, C. Y., Bindbeutel, R., Goldsworthy, J., Tielking, A., Alvarez, S., ... Nusinow, D. A. (2016). PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis. eLife, 5(FEBRUARY2016), [e13292]. https://doi.org/10.7554/eLife.13292

PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis. / Huang, He; Yoo, Chan Yul; Bindbeutel, Rebecca; Goldsworthy, Jessica; Tielking, Allison; Alvarez, Sophie; Naldrett, Michael J.; Evans, Bradley S.; Chen, Meng; Nusinow, Dmitri A.

In: eLife, Vol. 5, No. FEBRUARY2016, e13292, 03.02.2016.

Research output: Contribution to journalArticle

Huang, H, Yoo, CY, Bindbeutel, R, Goldsworthy, J, Tielking, A, Alvarez, S, Naldrett, MJ, Evans, BS, Chen, M & Nusinow, DA 2016, 'PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis', eLife, vol. 5, no. FEBRUARY2016, e13292. https://doi.org/10.7554/eLife.13292
Huang, He ; Yoo, Chan Yul ; Bindbeutel, Rebecca ; Goldsworthy, Jessica ; Tielking, Allison ; Alvarez, Sophie ; Naldrett, Michael J. ; Evans, Bradley S. ; Chen, Meng ; Nusinow, Dmitri A. / PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis. In: eLife. 2016 ; Vol. 5, No. FEBRUARY2016.
@article{7ac3f6ab02944b38ba01c755eacc90bf,
title = "PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis",
abstract = "Plants react to seasonal change in day length through altering physiology and development. Factors that function to harmonize growth with photoperiod are poorly understood. Here we characterize a new protein that associates with both circadian clock and photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens hypocotyls independent of day length. PCH1 peaks at dusk, binds phytochrome B (phyB) in a red light-dependent manner, and co-localizes with phyB into photobodies. PCH1 is necessary and sufficient to promote the biogenesis of large photobodies to maintain an active phyB pool after light exposure, potentiating red-light signaling and prolonging memory of prior illumination. Manipulating PCH1 alters PHYTOCHROME INTERACTING FACTOR 4 levels and regulates light-responsive gene expression. Thus, PCH1 is a new factor that regulates photoperiod-responsive growth by integrating the clock with light perception pathways through modulating daily phyB-signaling.",
author = "He Huang and Yoo, {Chan Yul} and Rebecca Bindbeutel and Jessica Goldsworthy and Allison Tielking and Sophie Alvarez and Naldrett, {Michael J.} and Evans, {Bradley S.} and Meng Chen and Nusinow, {Dmitri A.}",
year = "2016",
month = "2",
day = "3",
doi = "10.7554/eLife.13292",
language = "English (US)",
volume = "5",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
number = "FEBRUARY2016",

}

TY - JOUR

T1 - PCH1 integrates circadian and light-signaling pathways to control photoperiod-responsive growth in Arabidopsis

AU - Huang, He

AU - Yoo, Chan Yul

AU - Bindbeutel, Rebecca

AU - Goldsworthy, Jessica

AU - Tielking, Allison

AU - Alvarez, Sophie

AU - Naldrett, Michael J.

AU - Evans, Bradley S.

AU - Chen, Meng

AU - Nusinow, Dmitri A.

PY - 2016/2/3

Y1 - 2016/2/3

N2 - Plants react to seasonal change in day length through altering physiology and development. Factors that function to harmonize growth with photoperiod are poorly understood. Here we characterize a new protein that associates with both circadian clock and photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens hypocotyls independent of day length. PCH1 peaks at dusk, binds phytochrome B (phyB) in a red light-dependent manner, and co-localizes with phyB into photobodies. PCH1 is necessary and sufficient to promote the biogenesis of large photobodies to maintain an active phyB pool after light exposure, potentiating red-light signaling and prolonging memory of prior illumination. Manipulating PCH1 alters PHYTOCHROME INTERACTING FACTOR 4 levels and regulates light-responsive gene expression. Thus, PCH1 is a new factor that regulates photoperiod-responsive growth by integrating the clock with light perception pathways through modulating daily phyB-signaling.

AB - Plants react to seasonal change in day length through altering physiology and development. Factors that function to harmonize growth with photoperiod are poorly understood. Here we characterize a new protein that associates with both circadian clock and photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens hypocotyls independent of day length. PCH1 peaks at dusk, binds phytochrome B (phyB) in a red light-dependent manner, and co-localizes with phyB into photobodies. PCH1 is necessary and sufficient to promote the biogenesis of large photobodies to maintain an active phyB pool after light exposure, potentiating red-light signaling and prolonging memory of prior illumination. Manipulating PCH1 alters PHYTOCHROME INTERACTING FACTOR 4 levels and regulates light-responsive gene expression. Thus, PCH1 is a new factor that regulates photoperiod-responsive growth by integrating the clock with light perception pathways through modulating daily phyB-signaling.

UR - http://www.scopus.com/inward/record.url?scp=84961904385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961904385&partnerID=8YFLogxK

U2 - 10.7554/eLife.13292

DO - 10.7554/eLife.13292

M3 - Article

C2 - 26839287

AN - SCOPUS:84961904385

VL - 5

JO - eLife

JF - eLife

SN - 2050-084X

IS - FEBRUARY2016

M1 - e13292

ER -