Patterns of Nutrient Uptake by the Mammary Glands of Lactating Dairy Cows

P. S. Miller, B. L. Reis, C. C. Calvert, E. J. DePeters, R. L. Baldwin

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Abstract

Twenty-one multiparous lactating dairy cows with previous 305-d milk production records varying from 5900 to 13,600 kg were used to examine patterns of nutrient uptake by the mammary glands. On d 71 and continuing until d 126 of lactation, animals were injected daily with 40 mg of sometribove (bST group) or bicarbonate buffer (control group). Arterial and venous blood plasma samples were collected over a 12-h period on d 35, 70, 105, and 126 of lactation. Regression equations developed to evaluate linear effects of plasma arterial concentrations on net arterial-venous difference across the mammary glands demonstrated that, for acetate, NEFA, and D-β-hydroxybutyrate, plasma arterial concentration accounted for over 50% of variation in uptake by the mammary glands. Additionally, a sigmoidal equation fitted the relationship between D-β-hydroxybutyrate plasma arterial concentration and mammary gland uptake (r2 = .70). Triacylglyceride concentration was less effective in predicting uptake (r2 = .25). Administration of bST did not alter patterns of nutrient uptake, but a fourfold increase in NEFA uptake was predicted for bST-treated cows from this study, using NEFA concentrations from the literature. These observations indicate that plasma concentrations of acetate, NEFA, D-β-hydroxybutyrate, and triacylglyceride are major determinants of uptake by the mammary glands. Factors other than plasma concentration, such as mammary gland biosynthetic capacity, availability of other nutrients, and blood flow, determine uptakes of glucose, lactate, and total and free cholesterol (r2 ≤ .03).

Original languageEnglish (US)
Pages (from-to)3791-3799
Number of pages9
JournalJournal of Dairy Science
Volume74
Issue number11
DOIs
StatePublished - Jan 1 1991

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Human Mammary Glands
mammary glands
nutrient uptake
dairy cows
Hydroxybutyrates
uptake mechanisms
Nonesterified Fatty Acids
Lactation
Acetates
lactation
acetates
Bicarbonates
blood plasma
bicarbonates
nutrient availability
blood flow
lactates
Lactic Acid
milk production
Buffers

Keywords

  • AVD
  • CoA
  • D-β-hydroxybutyrate
  • FCHOL
  • K
  • PAC
  • T
  • TAG
  • TCHOL
  • V
  • arterial-venous difference
  • coenzyme A
  • dairy cow
  • free cholesterol
  • mammary glands
  • nutrient uptake
  • plasma arterial concentration
  • sigmoidicity parameter
  • theoretical estimate for maximum AVD at infinite βHBA PAC
  • total cholesterol
  • triacylglyceride
  • βHBA
  • βHBA PAC at which one-half V is obtained

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Patterns of Nutrient Uptake by the Mammary Glands of Lactating Dairy Cows. / Miller, P. S.; Reis, B. L.; Calvert, C. C.; DePeters, E. J.; Baldwin, R. L.

In: Journal of Dairy Science, Vol. 74, No. 11, 01.01.1991, p. 3791-3799.

Research output: Contribution to journalArticle

Miller, P. S. ; Reis, B. L. ; Calvert, C. C. ; DePeters, E. J. ; Baldwin, R. L. / Patterns of Nutrient Uptake by the Mammary Glands of Lactating Dairy Cows. In: Journal of Dairy Science. 1991 ; Vol. 74, No. 11. pp. 3791-3799.
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