Patterns of gene expression in Western corn rootworm (Diabrotica virgifera virgifera) neonates, challenged with Cry34ab1, Cry35Ab1 and Cry34/35Ab1, based on next‐generation sequencing

Haichuan Wang, Seong‐Il Eyun, Kanika Arora, Sek Yee Tan, Premchand Gandra, Etsuko Moriyama, Chitvan Khajuria, Jessica Jurzenski, Huarong Li, Maia Donahue, Ken Narva, Blair Siegfried

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

With Next Generation Sequencing technologies, high‐throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified in DETs in this study. The majority of DETs (75% with Cry34Ab1 and 68.3% with Cry34/35Ab1 treatments) had no significant hits in the NCBI nr database. In addition, 92 DETs were shared between Cry34Ab1 and Cry34/35Ab1 treatments. Further analysis revealed that the most abundant DETs in both Cry34Ab1 and Cry34/35Ab1 treatments were associated with binding and catalytic activity. Results from this study confirmed the nature of these binary toxins against WCR larvae and provide a fundamental profile of expression pattern of genes in response to challenge of the Cry34/35Ab1 toxin, which may provide insight into potential resistance mechanisms.

Original languageEnglish (US)
Article number124
JournalToxins
Volume9
Issue number4
DOIs
StatePublished - Apr 2017

Fingerprint

Gene expression
Zea mays
Larva
RNA Sequence Analysis
Gene Expression
Bacillus thuringiensis
Bacilli
Transcriptome
Insects
Catalyst activity
Proteins
Genes
Databases
RNA
Technology

Keywords

  • Bt challenge
  • Diabrotica virgifera virgifera
  • Differential gene expression
  • Next generation sequencing (NGS)

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Patterns of gene expression in Western corn rootworm (Diabrotica virgifera virgifera) neonates, challenged with Cry34ab1, Cry35Ab1 and Cry34/35Ab1, based on next‐generation sequencing. / Wang, Haichuan; Eyun, Seong‐Il; Arora, Kanika; Tan, Sek Yee; Gandra, Premchand; Moriyama, Etsuko; Khajuria, Chitvan; Jurzenski, Jessica; Li, Huarong; Donahue, Maia; Narva, Ken; Siegfried, Blair.

In: Toxins, Vol. 9, No. 4, 124, 04.2017.

Research output: Contribution to journalArticle

Wang, Haichuan ; Eyun, Seong‐Il ; Arora, Kanika ; Tan, Sek Yee ; Gandra, Premchand ; Moriyama, Etsuko ; Khajuria, Chitvan ; Jurzenski, Jessica ; Li, Huarong ; Donahue, Maia ; Narva, Ken ; Siegfried, Blair. / Patterns of gene expression in Western corn rootworm (Diabrotica virgifera virgifera) neonates, challenged with Cry34ab1, Cry35Ab1 and Cry34/35Ab1, based on next‐generation sequencing. In: Toxins. 2017 ; Vol. 9, No. 4.
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abstract = "With Next Generation Sequencing technologies, high‐throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified in DETs in this study. The majority of DETs (75{\%} with Cry34Ab1 and 68.3{\%} with Cry34/35Ab1 treatments) had no significant hits in the NCBI nr database. In addition, 92 DETs were shared between Cry34Ab1 and Cry34/35Ab1 treatments. Further analysis revealed that the most abundant DETs in both Cry34Ab1 and Cry34/35Ab1 treatments were associated with binding and catalytic activity. Results from this study confirmed the nature of these binary toxins against WCR larvae and provide a fundamental profile of expression pattern of genes in response to challenge of the Cry34/35Ab1 toxin, which may provide insight into potential resistance mechanisms.",
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AU - Wang, Haichuan

AU - Eyun, Seong‐Il

AU - Arora, Kanika

AU - Tan, Sek Yee

AU - Gandra, Premchand

AU - Moriyama, Etsuko

AU - Khajuria, Chitvan

AU - Jurzenski, Jessica

AU - Li, Huarong

AU - Donahue, Maia

AU - Narva, Ken

AU - Siegfried, Blair

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N2 - With Next Generation Sequencing technologies, high‐throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified in DETs in this study. The majority of DETs (75% with Cry34Ab1 and 68.3% with Cry34/35Ab1 treatments) had no significant hits in the NCBI nr database. In addition, 92 DETs were shared between Cry34Ab1 and Cry34/35Ab1 treatments. Further analysis revealed that the most abundant DETs in both Cry34Ab1 and Cry34/35Ab1 treatments were associated with binding and catalytic activity. Results from this study confirmed the nature of these binary toxins against WCR larvae and provide a fundamental profile of expression pattern of genes in response to challenge of the Cry34/35Ab1 toxin, which may provide insight into potential resistance mechanisms.

AB - With Next Generation Sequencing technologies, high‐throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified in DETs in this study. The majority of DETs (75% with Cry34Ab1 and 68.3% with Cry34/35Ab1 treatments) had no significant hits in the NCBI nr database. In addition, 92 DETs were shared between Cry34Ab1 and Cry34/35Ab1 treatments. Further analysis revealed that the most abundant DETs in both Cry34Ab1 and Cry34/35Ab1 treatments were associated with binding and catalytic activity. Results from this study confirmed the nature of these binary toxins against WCR larvae and provide a fundamental profile of expression pattern of genes in response to challenge of the Cry34/35Ab1 toxin, which may provide insight into potential resistance mechanisms.

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KW - Differential gene expression

KW - Next generation sequencing (NGS)

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