Patterning mechanisms of cytoskeletal and cell wall systems during leaf trichome morphogenesis

Makoto Yanagisawa, Anastasia S. Desyatova, Samuel A. Belteton, Eileen L. Mallery, Joseph A Turner, Daniel B. Szymanski

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The plant actin cytoskeleton is an unstable network of filaments that influences polarized growth through poorly understood mechanisms. Here, we used a combination of live cell imaging and finite element computational modelling of Arabidopsis trichome morphogenesis to determine how the actin and microtubule cytoskeletons cooperate to pattern the cell wall and growth. The actin-related protein (ARP)2/3 complex generates an actin meshwork that operates within a tip-localized, microtubule-depleted zone to modulate cell wall anisotropy locally. The actin meshwork also positions an actin bundle network that organizes organelle flow patterns. This activity is required to maintain cell wall thickness gradients that enable tip-biased diffuse growth. These newly discovered couplings between cytoskeletal patterns and wall textures provide important insights into the cellular mechanism of growth control in plants.

Original languageEnglish (US)
Article number15014
JournalNature Plants
Volume1
DOIs
StatePublished - Mar 2 2015

Fingerprint

trichomes
morphogenesis
actin
cell walls
leaves
microtubules
plant growth control
cytoskeleton
microfilaments
organelles
cell growth
texture
Arabidopsis
image analysis
proteins
cells

ASJC Scopus subject areas

  • Plant Science

Cite this

Yanagisawa, M., Desyatova, A. S., Belteton, S. A., Mallery, E. L., Turner, J. A., & Szymanski, D. B. (2015). Patterning mechanisms of cytoskeletal and cell wall systems during leaf trichome morphogenesis. Nature Plants, 1, [15014]. https://doi.org/10.1038/nplants.2015.14

Patterning mechanisms of cytoskeletal and cell wall systems during leaf trichome morphogenesis. / Yanagisawa, Makoto; Desyatova, Anastasia S.; Belteton, Samuel A.; Mallery, Eileen L.; Turner, Joseph A; Szymanski, Daniel B.

In: Nature Plants, Vol. 1, 15014, 02.03.2015.

Research output: Contribution to journalArticle

Yanagisawa, Makoto ; Desyatova, Anastasia S. ; Belteton, Samuel A. ; Mallery, Eileen L. ; Turner, Joseph A ; Szymanski, Daniel B. / Patterning mechanisms of cytoskeletal and cell wall systems during leaf trichome morphogenesis. In: Nature Plants. 2015 ; Vol. 1.
@article{3cda4a43b4e14a009a4d34c912294da6,
title = "Patterning mechanisms of cytoskeletal and cell wall systems during leaf trichome morphogenesis",
abstract = "The plant actin cytoskeleton is an unstable network of filaments that influences polarized growth through poorly understood mechanisms. Here, we used a combination of live cell imaging and finite element computational modelling of Arabidopsis trichome morphogenesis to determine how the actin and microtubule cytoskeletons cooperate to pattern the cell wall and growth. The actin-related protein (ARP)2/3 complex generates an actin meshwork that operates within a tip-localized, microtubule-depleted zone to modulate cell wall anisotropy locally. The actin meshwork also positions an actin bundle network that organizes organelle flow patterns. This activity is required to maintain cell wall thickness gradients that enable tip-biased diffuse growth. These newly discovered couplings between cytoskeletal patterns and wall textures provide important insights into the cellular mechanism of growth control in plants.",
author = "Makoto Yanagisawa and Desyatova, {Anastasia S.} and Belteton, {Samuel A.} and Mallery, {Eileen L.} and Turner, {Joseph A} and Szymanski, {Daniel B.}",
year = "2015",
month = "3",
day = "2",
doi = "10.1038/nplants.2015.14",
language = "English (US)",
volume = "1",
journal = "Nature Plants",
issn = "2055-026X",
publisher = "Palgrave Macmillan Ltd.",

}

TY - JOUR

T1 - Patterning mechanisms of cytoskeletal and cell wall systems during leaf trichome morphogenesis

AU - Yanagisawa, Makoto

AU - Desyatova, Anastasia S.

AU - Belteton, Samuel A.

AU - Mallery, Eileen L.

AU - Turner, Joseph A

AU - Szymanski, Daniel B.

PY - 2015/3/2

Y1 - 2015/3/2

N2 - The plant actin cytoskeleton is an unstable network of filaments that influences polarized growth through poorly understood mechanisms. Here, we used a combination of live cell imaging and finite element computational modelling of Arabidopsis trichome morphogenesis to determine how the actin and microtubule cytoskeletons cooperate to pattern the cell wall and growth. The actin-related protein (ARP)2/3 complex generates an actin meshwork that operates within a tip-localized, microtubule-depleted zone to modulate cell wall anisotropy locally. The actin meshwork also positions an actin bundle network that organizes organelle flow patterns. This activity is required to maintain cell wall thickness gradients that enable tip-biased diffuse growth. These newly discovered couplings between cytoskeletal patterns and wall textures provide important insights into the cellular mechanism of growth control in plants.

AB - The plant actin cytoskeleton is an unstable network of filaments that influences polarized growth through poorly understood mechanisms. Here, we used a combination of live cell imaging and finite element computational modelling of Arabidopsis trichome morphogenesis to determine how the actin and microtubule cytoskeletons cooperate to pattern the cell wall and growth. The actin-related protein (ARP)2/3 complex generates an actin meshwork that operates within a tip-localized, microtubule-depleted zone to modulate cell wall anisotropy locally. The actin meshwork also positions an actin bundle network that organizes organelle flow patterns. This activity is required to maintain cell wall thickness gradients that enable tip-biased diffuse growth. These newly discovered couplings between cytoskeletal patterns and wall textures provide important insights into the cellular mechanism of growth control in plants.

UR - http://www.scopus.com/inward/record.url?scp=84990981030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990981030&partnerID=8YFLogxK

U2 - 10.1038/nplants.2015.14

DO - 10.1038/nplants.2015.14

M3 - Article

C2 - 27246881

AN - SCOPUS:84990981030

VL - 1

JO - Nature Plants

JF - Nature Plants

SN - 2055-026X

M1 - 15014

ER -