12 Citations (Scopus)

Abstract

Exposure to agriculture organic dusts, comprised of a diversity of pathogen-associated molecular patterns, results in chronic airway diseases. The multi-functional class A macrophage scavenger receptor (SRA)/CD204 has emerged as an important class of pattern recognition receptors with broad ligand binding ability. The objective was to determine the role of SRA in mediating repetitive and post-inflammatory organic dust extract (ODE)-induced airway inflammation. Wild-type (WT) and SRA knockout (KO) mice were intra-nasally treated with ODE or saline daily for 3 weeks and immediately euthanized or allowed to recover for 1 week. Results show that lung histopathologic changes were increased in SRA KO mice as compared to WT following repetitive ODE exposures marked predominately by increased size and distribution of lymphoid aggregates. After a 1-week recovery from daily ODE treatments, there was significant resolution of lung injury in WT mice, but not SRA KO animals. The increased lung histopathology induced by ODE treatment was associated with decreased accumulation of neutrophils, but greater accumulation of CD4+ T-cells. The lung cytokine milieu induced by ODE was consistent with a TH1/TH17 polarization in both WT and SRA KO mice. Overall, the data demonstrate that SRA/CD204 plays an important role in the normative inflammatory lung response to ODE, as evidenced by the enhanced dust-mediated injury viewed in the absence of this receptor.

Original languageEnglish (US)
Pages (from-to)64-73
Number of pages10
JournalJournal of Immunotoxicology
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Pattern Recognition Receptors
Scavenger Receptors
Dust
Pattern recognition
Homeostasis
Knockout Mice
Lung
Class A Scavenger Receptors
Organic Agriculture
T-cells
Lung Injury
Agriculture
Animals
Neutrophils
Chronic Disease
Polarization
Cytokines
Ligands
Inflammation
T-Lymphocytes

Keywords

  • Farm
  • Inflammation
  • Lung
  • Lymphocyte
  • TH17
  • Toll-like receptor

ASJC Scopus subject areas

  • Immunology
  • Toxicology

Cite this

Pattern recognition scavenger receptor A/CD204 regulates airway inflammatory homeostasis following organic dust extract exposures. / Poole, Jill A; Anderson, Leigh; Gleason, Angela M.; West, William W.; Romberger, Debra; Wyatt, Todd A.

In: Journal of Immunotoxicology, Vol. 12, No. 1, 01.01.2015, p. 64-73.

Research output: Contribution to journalArticle

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abstract = "Exposure to agriculture organic dusts, comprised of a diversity of pathogen-associated molecular patterns, results in chronic airway diseases. The multi-functional class A macrophage scavenger receptor (SRA)/CD204 has emerged as an important class of pattern recognition receptors with broad ligand binding ability. The objective was to determine the role of SRA in mediating repetitive and post-inflammatory organic dust extract (ODE)-induced airway inflammation. Wild-type (WT) and SRA knockout (KO) mice were intra-nasally treated with ODE or saline daily for 3 weeks and immediately euthanized or allowed to recover for 1 week. Results show that lung histopathologic changes were increased in SRA KO mice as compared to WT following repetitive ODE exposures marked predominately by increased size and distribution of lymphoid aggregates. After a 1-week recovery from daily ODE treatments, there was significant resolution of lung injury in WT mice, but not SRA KO animals. The increased lung histopathology induced by ODE treatment was associated with decreased accumulation of neutrophils, but greater accumulation of CD4+ T-cells. The lung cytokine milieu induced by ODE was consistent with a TH1/TH17 polarization in both WT and SRA KO mice. Overall, the data demonstrate that SRA/CD204 plays an important role in the normative inflammatory lung response to ODE, as evidenced by the enhanced dust-mediated injury viewed in the absence of this receptor.",
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AU - Wyatt, Todd A

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