Pathogenicity of a bovine attaching effacing Escherichia coli isolate lacking Shiga-like toxins.

J. Fischer, C. Maddox, R. Moxley, D. Kinden, M. Miller

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Escherichia coli isolate 7996-90, obtained from a calf with diarrhea, had negative results of tests for K-88, K-99, 987P, F41, CS31A, F1845, F165 or E. coli adherence factor adhesins and had negative results of tests for the toxins heat-labile, heat-stable A, heat-stable B, Shiga-like toxin (SLT)-I or SLT-II. Strain 7996-90 had localized adherence to HEp-2 cells, caused actin rearrangement in host cells to which it adhered, hybridized with the eaeA probe, and produced the 94-kd outer membrane protein associated with attaching effacing lesions. This isolate caused attaching effacing lesions in Caco-2 cell polar monolayers, rabbit intestinal loops, and the intestines of gnotobiotic pigs. The isolate belongs to serotype O26: NM and is considered a class-II attaching effacing enteropathogenic E coli. Until recent addition of more sensitive assays at veterinary diagnostic laboratories, isolates such as 7996-90 were not readily recognized as pathogens because they failed to fit into the enterohemorrhagic E coli group, members of which, be definition, produce SLT. The assays described can facilitate diagnosis of attaching effacing E coli infection when histologic evaluation is hampered by autolysis.

Original languageEnglish (US)
Pages (from-to)991-999
Number of pages9
JournalAmerican journal of veterinary research
Volume55
Issue number7
StatePublished - Jul 1994

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Shiga-like toxins
Shiga Toxins
Virulence
pathogenicity
Hot Temperature
Escherichia coli
heat
lesions (animal)
cattle
Shiga Toxin 1
Shiga Toxin 2
Germ-Free Life
Enterohemorrhagic Escherichia coli
Enteropathogenic Escherichia coli
enteropathogenic Escherichia coli
Autolysis
Escherichia coli Infections
enterohemorrhagic Escherichia coli
adhesins
autolysis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Pathogenicity of a bovine attaching effacing Escherichia coli isolate lacking Shiga-like toxins. / Fischer, J.; Maddox, C.; Moxley, R.; Kinden, D.; Miller, M.

In: American journal of veterinary research, Vol. 55, No. 7, 07.1994, p. 991-999.

Research output: Contribution to journalArticle

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