PARylation of the forkhead-associated domain protein DAWDLE regulates plant immunity

Baomin Feng, Shisong Ma, Sixue Chen, Ning Zhu, Shuxin Zhang, Bin Yu, Yu Yu, Brandon Le, Xuemei Chen, Savithramma P. Dinesh-Kumar, Libo Shan, Ping He

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Protein poly(ADP-ribosyl)ation (PARylation) primarily catalyzed by poly(ADP-ribose) polymerases (PARPs) plays a crucial role in controlling various cellular responses. However, PARylation targets and their functions remain largely elusive. Here, we deployed an Arabidopsis protein microarray coupled with in vitro PARylation assays to globally identify PARylation targets in plants. Consistent with the essential role of PARylation in plant immunity, the forkhead-associated (FHA) domain protein DAWDLE (DDL), one of PARP2 targets, positively regulates plant defense to both adapted and non-adapted pathogens. Arabidopsis PARP2 interacts with and PARylates DDL, which was enhanced upon treatment of bacterial flagellin. Mass spectrometry and mutagenesis analysis identified multiple PARylation sites of DDL by PARP2. Genetic complementation assays indicate that DDL PARylation is required for its function in plant immunity. In contrast, DDL PARylation appears to be dispensable for its previously reported function in plant development partially mediated by the regulation of microRNA biogenesis. Our study uncovers many previously unknown PARylation targets and points to the distinct functions of DDL in plant immunity and development mediated by protein PARylation and small RNA biogenesis, respectively.

Original languageEnglish (US)
Pages (from-to)1799-1813
Number of pages15
JournalEMBO Reports
Volume17
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Plant Immunity
Adenosine Diphosphate
Proteins
Plant Development
Assays
Arabidopsis Proteins
Flagellin
Protein Array Analysis
Mutagenesis
Poly(ADP-ribose) Polymerases
Protein Domains
Pathogens
Microarrays
MicroRNAs
Arabidopsis
Mass spectrometry
Mass Spectrometry
RNA

Keywords

  • FHA domain protein
  • immune gene expression
  • plant immunity
  • protein poly(ADP-ribosyl)ation (PARylation)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

PARylation of the forkhead-associated domain protein DAWDLE regulates plant immunity. / Feng, Baomin; Ma, Shisong; Chen, Sixue; Zhu, Ning; Zhang, Shuxin; Yu, Bin; Yu, Yu; Le, Brandon; Chen, Xuemei; Dinesh-Kumar, Savithramma P.; Shan, Libo; He, Ping.

In: EMBO Reports, Vol. 17, No. 12, 01.12.2016, p. 1799-1813.

Research output: Contribution to journalArticle

Feng, B, Ma, S, Chen, S, Zhu, N, Zhang, S, Yu, B, Yu, Y, Le, B, Chen, X, Dinesh-Kumar, SP, Shan, L & He, P 2016, 'PARylation of the forkhead-associated domain protein DAWDLE regulates plant immunity', EMBO Reports, vol. 17, no. 12, pp. 1799-1813. https://doi.org/10.15252/embr.201642486
Feng, Baomin ; Ma, Shisong ; Chen, Sixue ; Zhu, Ning ; Zhang, Shuxin ; Yu, Bin ; Yu, Yu ; Le, Brandon ; Chen, Xuemei ; Dinesh-Kumar, Savithramma P. ; Shan, Libo ; He, Ping. / PARylation of the forkhead-associated domain protein DAWDLE regulates plant immunity. In: EMBO Reports. 2016 ; Vol. 17, No. 12. pp. 1799-1813.
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