Parvovirus b19 as an etiologic agent of fulminant non-a, non-b, non-c, non-e hepatms (hna-e): evidence for productive viral infection

S. J. Naides, W. Karetnvi, P. R. Beck, A. N. Lancinas, R. S. Markin

Research output: Contribution to journalArticle

Abstract

We previously found B19 DNA in liver tissue of HNA-E patients with or without red cell aplasia. In this study, we used immune adherence polymerase chain reaction (IA-PCR) to demonstrate productive infection by showing whole B19 viral particles in livers from the patients with fulminant hepatitis of unknown etiology. B19 virions were captured by monoclonal anti-B19 antibody bound to solid phase and standard PCR amplification was performed. IA-PCR had specificity and sensitivity comparable to standard PCR. Using IAPCR, B19 was detected in 5 of 6 (83%) livers from patients with fulminant HNA-E and aplastic anemia; in 3 of 4 (75%) with fulminant HNA-E only; 1 of 4 (25%) with liver transplant complications, and 6 of 34 (18%) patients with chronic liver diseases. Detection by IA-PCR of "full" B19 virions in the liver from the patients with fulminant HNA-E with or without aplastic anemia confirmed the presence of productive B19 infection, suggesting the direct involvement of B19 in the etiology and pathogenesis of fulminant hepatitis of unknown etiology.

Original languageEnglish (US)
Pages (from-to)A1003
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

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Parvovirus
Protoparvovirus
Virus Diseases
Liver
Polymerase Chain Reaction
virion
Virion
Polymerase chain reaction
etiology
liver
infection
Aplastic Anemia
polymerase chain reaction
hepatitis
anemia
Hepatitis
liver transplant
liver diseases
Transplants
Infection

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Parvovirus b19 as an etiologic agent of fulminant non-a, non-b, non-c, non-e hepatms (hna-e) : evidence for productive viral infection. / Naides, S. J.; Karetnvi, W.; Beck, P. R.; Lancinas, A. N.; Markin, R. S.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996, p. A1003.

Research output: Contribution to journalArticle

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