Particulate matter in cigarette smoke increases ciliary axoneme beating through mechanical stimulation

Chelsea R. Navarrette, Joseph Harold Sisson, Elizabeth Nance, Diane Allen-Gipson, Justin Hanes, Todd A Wyatt

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: The lung's ability to trap and clear foreign particles via the mucociliary elevator is an important mechanism for protecting the lung against respirable irritants and microorganisms. Although cigarette smoke (CS) exposure and particulate inhalation are known to alter mucociliary clearance, little is known about how CS and nanoparticles (NPs) modify cilia beating at the cytoskeletal infrastructure, or axonemal, level. Methods: We used a cell-free model to introduce cigarette smoke extract (CSE) and NPs with variant size and surface chemistry to isolated axonemes and measured changes in ciliary motility. We hypothesized that CSE would alter cilia beating and that alterations in ciliary beat frequency (CBF) due to particulate matter would be size- and surface chemistry-dependent. Demembranated axonemes were isolated from ciliated bovine tracheas and exposed to adenosine triphosphate (ATP) to initiate motility. CBF was measured in response to 5% CSE, CSE filtrate, and carboxyl-modified (COOH), sulphate (SO 4)-modified (sulfonated), or PEG-coated polystyrene (PS) latex NPs ranging in size from 40nm to 500 nm. Results: CSE concentrations as low as 5% resulted in rapid, significant stimulation of CBF ( p < 0.05 vs. baseline control). Filtering CSE through a 0.2-μm filter attenuated this effect. Introduction of sulphate-modified PS beads ∼300 nm in diameter resulted in a similar increase in CBF above baseline ATP levels. Uncharged, PEG-coated beads had no effect on CBF regardless of size. Similarly, COOH-coated particles less than 200nm in diameter did not alter ciliary motility. However, COOH-coated PS particles larger than 300nm increased CBF significantly and increased the number of motile points. Conclusions: These data show that NPs, including those found in CSE, mechanically stimulate axonemes in a size- and surface chemistry-dependent manner. Alterations in ciliary motility due to physicochemical properties of NPs may be important for inhalational lung injury and efficient drug delivery of respirable particles.

Original languageEnglish (US)
Pages (from-to)159-168
Number of pages10
JournalJournal of Aerosol Medicine and Pulmonary Drug Delivery
Volume25
Issue number3
DOIs
StatePublished - Jun 1 2012

Fingerprint

Axoneme
Particulate Matter
Smoke
Tobacco Products
Nanoparticles
Cilia
Adenosine Triphosphate
Elevators and Escalators
Mucociliary Clearance
Inhalation Exposure
Lung
Irritants
Polystyrenes
Lung Injury
Trachea
Sulfates

Keywords

  • cigarette smoke (CS)
  • cilia; axonemes
  • mucociliary clearance
  • nanoparticles
  • particulates
  • pulmonary delivery

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

Particulate matter in cigarette smoke increases ciliary axoneme beating through mechanical stimulation. / Navarrette, Chelsea R.; Sisson, Joseph Harold; Nance, Elizabeth; Allen-Gipson, Diane; Hanes, Justin; Wyatt, Todd A.

In: Journal of Aerosol Medicine and Pulmonary Drug Delivery, Vol. 25, No. 3, 01.06.2012, p. 159-168.

Research output: Contribution to journalArticle

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T1 - Particulate matter in cigarette smoke increases ciliary axoneme beating through mechanical stimulation

AU - Navarrette, Chelsea R.

AU - Sisson, Joseph Harold

AU - Nance, Elizabeth

AU - Allen-Gipson, Diane

AU - Hanes, Justin

AU - Wyatt, Todd A

PY - 2012/6/1

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N2 - Background: The lung's ability to trap and clear foreign particles via the mucociliary elevator is an important mechanism for protecting the lung against respirable irritants and microorganisms. Although cigarette smoke (CS) exposure and particulate inhalation are known to alter mucociliary clearance, little is known about how CS and nanoparticles (NPs) modify cilia beating at the cytoskeletal infrastructure, or axonemal, level. Methods: We used a cell-free model to introduce cigarette smoke extract (CSE) and NPs with variant size and surface chemistry to isolated axonemes and measured changes in ciliary motility. We hypothesized that CSE would alter cilia beating and that alterations in ciliary beat frequency (CBF) due to particulate matter would be size- and surface chemistry-dependent. Demembranated axonemes were isolated from ciliated bovine tracheas and exposed to adenosine triphosphate (ATP) to initiate motility. CBF was measured in response to 5% CSE, CSE filtrate, and carboxyl-modified (COOH), sulphate (SO 4)-modified (sulfonated), or PEG-coated polystyrene (PS) latex NPs ranging in size from 40nm to 500 nm. Results: CSE concentrations as low as 5% resulted in rapid, significant stimulation of CBF ( p < 0.05 vs. baseline control). Filtering CSE through a 0.2-μm filter attenuated this effect. Introduction of sulphate-modified PS beads ∼300 nm in diameter resulted in a similar increase in CBF above baseline ATP levels. Uncharged, PEG-coated beads had no effect on CBF regardless of size. Similarly, COOH-coated particles less than 200nm in diameter did not alter ciliary motility. However, COOH-coated PS particles larger than 300nm increased CBF significantly and increased the number of motile points. Conclusions: These data show that NPs, including those found in CSE, mechanically stimulate axonemes in a size- and surface chemistry-dependent manner. Alterations in ciliary motility due to physicochemical properties of NPs may be important for inhalational lung injury and efficient drug delivery of respirable particles.

AB - Background: The lung's ability to trap and clear foreign particles via the mucociliary elevator is an important mechanism for protecting the lung against respirable irritants and microorganisms. Although cigarette smoke (CS) exposure and particulate inhalation are known to alter mucociliary clearance, little is known about how CS and nanoparticles (NPs) modify cilia beating at the cytoskeletal infrastructure, or axonemal, level. Methods: We used a cell-free model to introduce cigarette smoke extract (CSE) and NPs with variant size and surface chemistry to isolated axonemes and measured changes in ciliary motility. We hypothesized that CSE would alter cilia beating and that alterations in ciliary beat frequency (CBF) due to particulate matter would be size- and surface chemistry-dependent. Demembranated axonemes were isolated from ciliated bovine tracheas and exposed to adenosine triphosphate (ATP) to initiate motility. CBF was measured in response to 5% CSE, CSE filtrate, and carboxyl-modified (COOH), sulphate (SO 4)-modified (sulfonated), or PEG-coated polystyrene (PS) latex NPs ranging in size from 40nm to 500 nm. Results: CSE concentrations as low as 5% resulted in rapid, significant stimulation of CBF ( p < 0.05 vs. baseline control). Filtering CSE through a 0.2-μm filter attenuated this effect. Introduction of sulphate-modified PS beads ∼300 nm in diameter resulted in a similar increase in CBF above baseline ATP levels. Uncharged, PEG-coated beads had no effect on CBF regardless of size. Similarly, COOH-coated particles less than 200nm in diameter did not alter ciliary motility. However, COOH-coated PS particles larger than 300nm increased CBF significantly and increased the number of motile points. Conclusions: These data show that NPs, including those found in CSE, mechanically stimulate axonemes in a size- and surface chemistry-dependent manner. Alterations in ciliary motility due to physicochemical properties of NPs may be important for inhalational lung injury and efficient drug delivery of respirable particles.

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