Participation of leaky ribosome scanning in protein dual targeting by alternative translation initiation in higher plants

Yashitola Wamboldt, Saleem Mohammed, Christian Elowsky, Chris Wittgren, Wilson B M De Paula, Sally Mackenzie

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Postendosymbiotic evolution has given rise to proteins that are multiply targeted within the cell. Various mechanisms have been identified to permit the expression of proteins encoding distinct N termini from a single gene. One mechanism involves alternative translation initiation (aTI). We previously showed evidence of aTI activity within the Arabidopsis thaliana organellar DNA polymerase gene POLγ2. Translation initiates at four distinct sites within this gene, two non-AUG, to produce distinct plastid and mitochondrially targeted forms of the protein. To understand the regulation of aTI in higher plants, we used Polγ2 as a model to investigate both cis- and trans-acting features of the process. Here, we show that aTI in Polγ2 and other plant genes involves ribosome scanning dependent on sequence context at the multiple initiation sites to condition specific binding of at least one trans-acting factor essential for site recognition. Multiple active translation initiation sites appear to operate in several plant genes, often to expand protein targeting. In plants, where the mitochondrion and plastid must share a considerable portion of their proteomes and coordinate their functions, leaky ribosome scanning behavior provides adaptive advantage in the evolution of protein dual targeting and translational regulation.

Original languageEnglish (US)
Pages (from-to)157-167
Number of pages11
JournalPlant Cell
Volume21
Issue number1
DOIs
StatePublished - Jan 1 2009

Fingerprint

Protein Transport
ribosomes
Ribosomes
translation (genetics)
Plant Genes
Plastids
Genes
Proteins
Trans-Activators
proteins
Psychological Adaptation
DNA-Directed DNA Polymerase
Proteome
genes
Arabidopsis
plastids
Mitochondria
DNA-directed DNA polymerase
proteome
mitochondria

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Wamboldt, Y., Mohammed, S., Elowsky, C., Wittgren, C., De Paula, W. B. M., & Mackenzie, S. (2009). Participation of leaky ribosome scanning in protein dual targeting by alternative translation initiation in higher plants. Plant Cell, 21(1), 157-167. https://doi.org/10.1105/tpc.108.063644

Participation of leaky ribosome scanning in protein dual targeting by alternative translation initiation in higher plants. / Wamboldt, Yashitola; Mohammed, Saleem; Elowsky, Christian; Wittgren, Chris; De Paula, Wilson B M; Mackenzie, Sally.

In: Plant Cell, Vol. 21, No. 1, 01.01.2009, p. 157-167.

Research output: Contribution to journalArticle

Wamboldt, Y, Mohammed, S, Elowsky, C, Wittgren, C, De Paula, WBM & Mackenzie, S 2009, 'Participation of leaky ribosome scanning in protein dual targeting by alternative translation initiation in higher plants', Plant Cell, vol. 21, no. 1, pp. 157-167. https://doi.org/10.1105/tpc.108.063644
Wamboldt, Yashitola ; Mohammed, Saleem ; Elowsky, Christian ; Wittgren, Chris ; De Paula, Wilson B M ; Mackenzie, Sally. / Participation of leaky ribosome scanning in protein dual targeting by alternative translation initiation in higher plants. In: Plant Cell. 2009 ; Vol. 21, No. 1. pp. 157-167.
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