Partial cloning and sequencing of chick fibrillin-1 cDNA

Guimei Zhou, Christopher E. Price, Thomas H. Rosenquist, Preston F. Gadson, Maurice Godfrey

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The recent identification of numerous matrix genes and gene products has allowed a detailed examination of their roles in development. Two of these extracellular matrix proteins, fibrillin-1 and fibrillin-2, are components of the elastin-associated microfibrils. Given what is known about the distribution of the fibrillins in normal tissues and the abnormalities that result when mutations occur, a basic hypothesis has emerged: fibrillin-1 is primarily responsible for load bearing and providing structural integrity, whereas fibrillin-2 may be a director of elastogenesis. Nevertheless, examination of phenotypes in disorders caused by mutations in fibrillin-1 or fibrillin-2 suggests some common functions. To better understand these similar and diverse roles, it would be helpful to examine these proteins during chick development. To accomplish this goal, it is first necessary to characterize the chick homologs of the known fibrillins. In this study, the partial chick FBN1 cDNA was identified by polymerase chain reaction-aided cloning as a first step toward elucidating these goals. Sequence analysis indicated that there is striking conservation between chick and mammalian fibrillin-1 at the DNA and protein levels. Antisense and sense riboprobes were synthesized and used in in situ hybridization in stage 14 chick embryos and high levels of FBN1 transcripts were observed in the heart.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume36
Issue number1
DOIs
StatePublished - Jan 1 2000

Fingerprint

Cloning
Organism Cloning
Bearings (structural)
Complementary DNA
Genes
Elastin
Extracellular Matrix Proteins
Polymerase chain reaction
Structural integrity
Conservation
Proteins
Tissue
Microfibrils
Mutation
DNA
Weight-Bearing
Chick Embryo
In Situ Hybridization
Sequence Analysis
Phenotype

Keywords

  • Chick
  • Development
  • Extracellular matrix
  • Fibrillin
  • In situ hybridization

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Partial cloning and sequencing of chick fibrillin-1 cDNA. / Zhou, Guimei; Price, Christopher E.; Rosenquist, Thomas H.; Gadson, Preston F.; Godfrey, Maurice.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 36, No. 1, 01.01.2000, p. 19-25.

Research output: Contribution to journalArticle

Zhou, Guimei ; Price, Christopher E. ; Rosenquist, Thomas H. ; Gadson, Preston F. ; Godfrey, Maurice. / Partial cloning and sequencing of chick fibrillin-1 cDNA. In: In Vitro Cellular and Developmental Biology - Animal. 2000 ; Vol. 36, No. 1. pp. 19-25.
@article{85a8e3d04e4e4823bbdfda5d7ca5afdb,
title = "Partial cloning and sequencing of chick fibrillin-1 cDNA",
abstract = "The recent identification of numerous matrix genes and gene products has allowed a detailed examination of their roles in development. Two of these extracellular matrix proteins, fibrillin-1 and fibrillin-2, are components of the elastin-associated microfibrils. Given what is known about the distribution of the fibrillins in normal tissues and the abnormalities that result when mutations occur, a basic hypothesis has emerged: fibrillin-1 is primarily responsible for load bearing and providing structural integrity, whereas fibrillin-2 may be a director of elastogenesis. Nevertheless, examination of phenotypes in disorders caused by mutations in fibrillin-1 or fibrillin-2 suggests some common functions. To better understand these similar and diverse roles, it would be helpful to examine these proteins during chick development. To accomplish this goal, it is first necessary to characterize the chick homologs of the known fibrillins. In this study, the partial chick FBN1 cDNA was identified by polymerase chain reaction-aided cloning as a first step toward elucidating these goals. Sequence analysis indicated that there is striking conservation between chick and mammalian fibrillin-1 at the DNA and protein levels. Antisense and sense riboprobes were synthesized and used in in situ hybridization in stage 14 chick embryos and high levels of FBN1 transcripts were observed in the heart.",
keywords = "Chick, Development, Extracellular matrix, Fibrillin, In situ hybridization",
author = "Guimei Zhou and Price, {Christopher E.} and Rosenquist, {Thomas H.} and Gadson, {Preston F.} and Maurice Godfrey",
year = "2000",
month = "1",
day = "1",
doi = "10.1290/1071-2690(2000)036<0019:PCASOC>2.3.CO;2",
language = "English (US)",
volume = "36",
pages = "19--25",
journal = "In Vitro Cellular and Developmental Biology - Plant",
issn = "1054-5476",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Partial cloning and sequencing of chick fibrillin-1 cDNA

AU - Zhou, Guimei

AU - Price, Christopher E.

AU - Rosenquist, Thomas H.

AU - Gadson, Preston F.

AU - Godfrey, Maurice

PY - 2000/1/1

Y1 - 2000/1/1

N2 - The recent identification of numerous matrix genes and gene products has allowed a detailed examination of their roles in development. Two of these extracellular matrix proteins, fibrillin-1 and fibrillin-2, are components of the elastin-associated microfibrils. Given what is known about the distribution of the fibrillins in normal tissues and the abnormalities that result when mutations occur, a basic hypothesis has emerged: fibrillin-1 is primarily responsible for load bearing and providing structural integrity, whereas fibrillin-2 may be a director of elastogenesis. Nevertheless, examination of phenotypes in disorders caused by mutations in fibrillin-1 or fibrillin-2 suggests some common functions. To better understand these similar and diverse roles, it would be helpful to examine these proteins during chick development. To accomplish this goal, it is first necessary to characterize the chick homologs of the known fibrillins. In this study, the partial chick FBN1 cDNA was identified by polymerase chain reaction-aided cloning as a first step toward elucidating these goals. Sequence analysis indicated that there is striking conservation between chick and mammalian fibrillin-1 at the DNA and protein levels. Antisense and sense riboprobes were synthesized and used in in situ hybridization in stage 14 chick embryos and high levels of FBN1 transcripts were observed in the heart.

AB - The recent identification of numerous matrix genes and gene products has allowed a detailed examination of their roles in development. Two of these extracellular matrix proteins, fibrillin-1 and fibrillin-2, are components of the elastin-associated microfibrils. Given what is known about the distribution of the fibrillins in normal tissues and the abnormalities that result when mutations occur, a basic hypothesis has emerged: fibrillin-1 is primarily responsible for load bearing and providing structural integrity, whereas fibrillin-2 may be a director of elastogenesis. Nevertheless, examination of phenotypes in disorders caused by mutations in fibrillin-1 or fibrillin-2 suggests some common functions. To better understand these similar and diverse roles, it would be helpful to examine these proteins during chick development. To accomplish this goal, it is first necessary to characterize the chick homologs of the known fibrillins. In this study, the partial chick FBN1 cDNA was identified by polymerase chain reaction-aided cloning as a first step toward elucidating these goals. Sequence analysis indicated that there is striking conservation between chick and mammalian fibrillin-1 at the DNA and protein levels. Antisense and sense riboprobes were synthesized and used in in situ hybridization in stage 14 chick embryos and high levels of FBN1 transcripts were observed in the heart.

KW - Chick

KW - Development

KW - Extracellular matrix

KW - Fibrillin

KW - In situ hybridization

UR - http://www.scopus.com/inward/record.url?scp=0034069759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034069759&partnerID=8YFLogxK

U2 - 10.1290/1071-2690(2000)036<0019:PCASOC>2.3.CO;2

DO - 10.1290/1071-2690(2000)036<0019:PCASOC>2.3.CO;2

M3 - Article

VL - 36

SP - 19

EP - 25

JO - In Vitro Cellular and Developmental Biology - Plant

JF - In Vitro Cellular and Developmental Biology - Plant

SN - 1054-5476

IS - 1

ER -