Partial characterization of a renotropic factor

D. S. Gaydos, H. Golclin, B. Jenson, D. Gersten, B. Boecleker, C. Bartz, Harry G. Preuss

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In 70 experiments, the existence of a circulating renal growth factor was confirmed by 9.3% stimulation of 3H-thymidine into the DNA of renal fragments incubating for 90 min in the presence of sera from 20-hour unilaterallly nephrectomized rats compared to sera from 20-hour sham-operated rats (p<0.001). Dialysis (7.4%, p<0.01) or removal of albumin (11.8%, p<0.001) from sera of both sham-operated and unilaterally nephrectomized rats did not appreciably change the magnitude of the statistically significant stimulation. When albumin-free sera were placed in boiling water for 1-3 min to coagulate protein, the stimulation was still significantly different from control (5%, p < 0.05). Addition of sera from unilaterally nephrectomized rats (20 h) to isolated nuclei, even isolated nuclei removed from growing kidneys (72 h after unilateral nephroctomy), faileds to enhance DNA synthesis significantly.

Original languageEnglish (US)
Pages (from-to)139-144
Number of pages6
JournalKidney and Blood Pressure Research
Volume6
Issue number3
DOIs
StatePublished - Jan 1 1983

Fingerprint

Serum
Kidney
DNA
Serum Albumin
Thymidine
Dialysis
Albumins
Intercellular Signaling Peptides and Proteins
renotropins
Water
Proteins

Keywords

  • DNA synthesis
  • Isolated nuclei
  • Partial characterization
  • Renal growth
  • Renotropin

ASJC Scopus subject areas

  • Nephrology
  • Cardiology and Cardiovascular Medicine

Cite this

Gaydos, D. S., Golclin, H., Jenson, B., Gersten, D., Boecleker, B., Bartz, C., & Preuss, H. G. (1983). Partial characterization of a renotropic factor. Kidney and Blood Pressure Research, 6(3), 139-144. https://doi.org/10.1159/000172892

Partial characterization of a renotropic factor. / Gaydos, D. S.; Golclin, H.; Jenson, B.; Gersten, D.; Boecleker, B.; Bartz, C.; Preuss, Harry G.

In: Kidney and Blood Pressure Research, Vol. 6, No. 3, 01.01.1983, p. 139-144.

Research output: Contribution to journalArticle

Gaydos, DS, Golclin, H, Jenson, B, Gersten, D, Boecleker, B, Bartz, C & Preuss, HG 1983, 'Partial characterization of a renotropic factor', Kidney and Blood Pressure Research, vol. 6, no. 3, pp. 139-144. https://doi.org/10.1159/000172892
Gaydos DS, Golclin H, Jenson B, Gersten D, Boecleker B, Bartz C et al. Partial characterization of a renotropic factor. Kidney and Blood Pressure Research. 1983 Jan 1;6(3):139-144. https://doi.org/10.1159/000172892
Gaydos, D. S. ; Golclin, H. ; Jenson, B. ; Gersten, D. ; Boecleker, B. ; Bartz, C. ; Preuss, Harry G. / Partial characterization of a renotropic factor. In: Kidney and Blood Pressure Research. 1983 ; Vol. 6, No. 3. pp. 139-144.
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