Paraventricular nucleus bicuculline alters frequency components of sympathetic nerve discharge bursts

M. J. Kenney, M. L. Weiss, K. P. Patel, Y. Wang, R. J. Fels

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Autospectral and coherence analyses were used to determine the effect of paraventricular nucleus (PVN) GABAA receptor antagonism [microinfusion or microinjections of bicuculline methiodide (BMI) 100 pmoles] on sympathetic nerve discharge (SND) frequency components (bursting pattern and relationships between discharges in regionally selective nerves) in α-chloralose-anesthetized rats. SND was recorded from the renal, splenic, and lumbar nerves. The following observations were made. First, PVN BMI microinjections, but not PVN saline or cortical BMI microinjections, transformed the cardiac-related SND bursting pattern in baroreceptor-innervated rats to one characterized by the presence of low-frequency bursts not synchronized to the cardiac cycle or phrenic nerve discharge bursts. Second, SND pattern changes were similar in the renal, splenic, and lumbar nerves, and peak coherence values relating low-frequency bursts in sympathetic nerve pairs (renal-splenic, renal-lumbar, and splenic-lumbar) were significantly increased from preinjection control after PVN BMI microinjection. Third, PVN BMI microinjections significantly increased the coupling between low-frequency SND bursts in baroreceptor-denervated rats. Finally, PVN BMI-induced changes in the SND bursting pattern were not observed after PVN pretreatment with muscimol (GABA agonist, 1 nmole). We conclude that PVN GABAA receptor antagonism profoundly alters the frequency components in sympathetic nerves.

Original languageEnglish (US)
Pages (from-to)H1233-H1241
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume281
Issue number3 50-3
StatePublished - Oct 1 2001

Fingerprint

Bicuculline
Paraventricular Hypothalamic Nucleus
Microinjections
Pressoreceptors
GABA-A Receptors
Kidney
GABA Agonists
Muscimol
Chloralose
Phrenic Nerve
bicuculline methiodide

Keywords

  • Autospectral analysis
  • Coherence function
  • Spragne-Dawley rats
  • Sympathetic nerve activity

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Paraventricular nucleus bicuculline alters frequency components of sympathetic nerve discharge bursts. / Kenney, M. J.; Weiss, M. L.; Patel, K. P.; Wang, Y.; Fels, R. J.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 281, No. 3 50-3, 01.10.2001, p. H1233-H1241.

Research output: Contribution to journalArticle

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