Parameters for optimizing detection of transforming growth factors

Angie Rizzino, Eric Ruff

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Transforming growth factors (TGF) induce the soft agar growth of nontransformed cells, such as NRK-49F cells. This report describes culture conditions that optimize the detection of TGF by NRK-49F cells. Two aspects are dealt with in depth: the need to select an appropriate clone of NRK-49F cells and the need to select a culture medium that supports little or no background growth in the absence of added TGF. Evidence is presented to demonstrate that medium supplemented with heat-treated, dialyzed bovine plasma provides better conditions for assaying TGF than serum-supplemented medium provides.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalJournal of Tissue Culture Methods
Volume10
Issue number2
DOIs
StatePublished - Jun 1986

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Keywords

  • NRK-49F cells
  • anchorage-independent growth
  • epidermal growth factor
  • plasma
  • soft agar growth
  • transforming growth factors

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

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