Parameters affecting attachment of Bacillus thuringiensis var. israelensis toxin to latex beads

Graham A. Couche, Mary Ann Pfannenstiel, Kenneth W. Nickerson

Research output: Contribution to journalArticle

Abstract

Bacillus thuringiensis var. israelensis protein toxin radiolabeled with14C-iodoacetamide was bound to latex beads. The efficiency of attachment was not affected by the temperature or length of incubation or by the presence of several different buffers, salts, or organic solvents. However, attachment decreased dramatically at pH values >8.5 or in the presence of detergents (anionic, neutral, or cationic). Protein concentrations ≥2-3 mg/m2 of bead surface led to a progressively decreasing efficiency of protein adsorption. With minor variations, these findings should also be applicable to the attachment of numerous other proteins of diagnostic significance.

Original languageEnglish (US)
Pages (from-to)128-133
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume24
Issue number2
DOIs
Publication statusPublished - May 1 1986

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ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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