Paramecium bursaria chlorella virus 1 proteome reveals novel architectural and regulatory features of a giant virus

David D. Dunigan, Ronald L. Cerny, Andrew T. Bauman, Jared C. Roach, Leslie C. Lane, Irina V. Agarkova, Kurt Wulser, Giane M. Yanai-Balser, James R. Gurnon, Jason C. Vitek, Bernard J. Kronschnabel, Adrien Jeanniard, Guillaume Blanc, Chris Upton, Garry A. Duncan, O. William McClung, Fangrui Ma, James L. Van Etten

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The 331-kbp chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) genome was resequenced and annotated to correct errors in the original 15-year-old sequence; 40 codons was considered the minimum protein size of an open reading frame. PBCV-1 has 416 predicted protein-encoding sequences and 11 tRNAs. A proteome analysis was also conducted on highly purified PBCV-1 virions using two mass spectrometry-based protocols. The mass spectrometry-derived data were compared to PBCV-1 and its host Chlorella variabilis NC64A predicted proteomes. Combined, these analyses revealed 148 unique virus-encoded proteins associated with the virion (about 35% of the coding capacity of the virus) and 1 host protein. Some of these proteins appear to be structural/architectural, whereas others have enzymatic, chromatin modification, and signal transduction functions. Most (106) of the proteins have no known function or homologs in the existing gene databases except as orthologs with proteins of other chloroviruses, phycodnaviruses, and nuclear-cytoplasmic large DNA viruses. The genes encoding these proteins are dispersed throughout the virus genome, and most are transcribed late or early-late in the infection cycle, which is consistent with virion morphogenesis.

Original languageEnglish (US)
Pages (from-to)8821-8834
Number of pages14
JournalJournal of virology
Volume86
Issue number16
DOIs
StatePublished - Aug 1 2012

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Dunigan, D. D., Cerny, R. L., Bauman, A. T., Roach, J. C., Lane, L. C., Agarkova, I. V., Wulser, K., Yanai-Balser, G. M., Gurnon, J. R., Vitek, J. C., Kronschnabel, B. J., Jeanniard, A., Blanc, G., Upton, C., Duncan, G. A., McClung, O. W., Ma, F., & Van Etten, J. L. (2012). Paramecium bursaria chlorella virus 1 proteome reveals novel architectural and regulatory features of a giant virus. Journal of virology, 86(16), 8821-8834. https://doi.org/10.1128/JVI.00907-12