Paramecium bursaria chlorella virus 1 encodes a polyamine acetyltransferase

Zachary Charlop-Powers, Jean Jakoncic, James R. Gurnon, James L. Van Etten, Ming Ming Zhou

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Paramecium bursaria chlorella virus 1 (PBCV-1), a large DNA virus that infects green algae, encodes a histone H3 lysine 27-specific methyltransferase that functions in global transcriptional silencing of the host. PBCV-1 has another gene a654l that encodes a protein with sequence similarity to the GCN5 family histone acetyltransferases. In this study, we report a 1.5 Å crystal structure of PBCV-1 A654L in a complex with coenzyme A. The structure reveals a unique feature of A654L that precludes its acetylation of histone peptide substrates. We demonstrate that A654L, hence named viral polyamine acetyltransferase (vPAT), acetylates polyamines such as putrescine, spermidine, cadaverine, and homospermidine present in both PBCV-1 and its host through a reaction dependent upon a conserved glutamate 27. Our study suggests that as the first virally encoded polyamine acetyltransferase, vPAT plays a possible key role in the regulation of polyamine catabolism in the host during viral replication.

Original languageEnglish (US)
Pages (from-to)9547-9551
Number of pages5
JournalJournal of Biological Chemistry
Volume287
Issue number12
DOIs
StatePublished - Mar 16 2012

Fingerprint

Paramecium
Chlorella
Acetyltransferases
Polyamines
Viruses
Histones
Cadaverine
Histone Acetyltransferases
Acetylation
Chlorophyta
DNA Viruses
Putrescine
Spermidine
Methyltransferases
Coenzyme A
Algae
Lysine
Glutamic Acid
Genes
Crystal structure

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Paramecium bursaria chlorella virus 1 encodes a polyamine acetyltransferase. / Charlop-Powers, Zachary; Jakoncic, Jean; Gurnon, James R.; Van Etten, James L.; Zhou, Ming Ming.

In: Journal of Biological Chemistry, Vol. 287, No. 12, 16.03.2012, p. 9547-9551.

Research output: Contribution to journalArticle

Charlop-Powers, Zachary ; Jakoncic, Jean ; Gurnon, James R. ; Van Etten, James L. ; Zhou, Ming Ming. / Paramecium bursaria chlorella virus 1 encodes a polyamine acetyltransferase. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 12. pp. 9547-9551.
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