Pancreatic expression of keratinocyte growth factor leads to differentiation of islet hepatocytes and proliferation of duct cells

Michelle L. Krakowski, Marcie R. Kritzik, Ellene M. Jones, Troy Krahl, Jae Lee, Marc Arnush, Danling Gu, Nora Sarvetnick

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Keratinocyte growth factor, (KGF), a member of the fibroblast growth factor (FGF) family, is involved in wound healing. It also promotes the differentiation of many epithelial tissues and proliferation of epithelial cells as well as pancreatic duct cells. Additionally, many members of the highly homologous FGF family (including KGF), influence both growth and cellular morphology in the developing embryo. We have previously observed elevated levels of KGF in our interferon-γ transgenic mouse model of pancreatic regeneration. To understand the role of KGF in pancreatic differentiation, we generated insulin promoter-regulated KGF transgenic mice. Remarkably, we have found that ectopic KGF expression resulted in the emergence of hepatocytes within the islets of Langerhans in the pancreas. Additionally, significant intra-islet duct cell proliferation in the pancreata of transgenic KGF mice was observed. The unexpected appearance of hepatocytes and proliferation of intra-islet duct cells in the pancreata of these mice evidently stemmed directly from local exposure to KGF.

Original languageEnglish (US)
Pages (from-to)683-691
Number of pages9
JournalAmerican Journal of Pathology
Volume154
Issue number3
DOIs
StatePublished - Mar 1999

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Fibroblast Growth Factor 7
Hepatocytes
Cell Proliferation
Islets of Langerhans
Pancreas
Fibroblast Growth Factors
Transgenic Mice
Pancreatic Ducts
Wound Healing
Interferons
Regeneration
Embryonic Structures
Epithelium
Epithelial Cells
Insulin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Pancreatic expression of keratinocyte growth factor leads to differentiation of islet hepatocytes and proliferation of duct cells. / Krakowski, Michelle L.; Kritzik, Marcie R.; Jones, Ellene M.; Krahl, Troy; Lee, Jae; Arnush, Marc; Gu, Danling; Sarvetnick, Nora.

In: American Journal of Pathology, Vol. 154, No. 3, 03.1999, p. 683-691.

Research output: Contribution to journalArticle

Krakowski, Michelle L. ; Kritzik, Marcie R. ; Jones, Ellene M. ; Krahl, Troy ; Lee, Jae ; Arnush, Marc ; Gu, Danling ; Sarvetnick, Nora. / Pancreatic expression of keratinocyte growth factor leads to differentiation of islet hepatocytes and proliferation of duct cells. In: American Journal of Pathology. 1999 ; Vol. 154, No. 3. pp. 683-691.
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abstract = "Keratinocyte growth factor, (KGF), a member of the fibroblast growth factor (FGF) family, is involved in wound healing. It also promotes the differentiation of many epithelial tissues and proliferation of epithelial cells as well as pancreatic duct cells. Additionally, many members of the highly homologous FGF family (including KGF), influence both growth and cellular morphology in the developing embryo. We have previously observed elevated levels of KGF in our interferon-γ transgenic mouse model of pancreatic regeneration. To understand the role of KGF in pancreatic differentiation, we generated insulin promoter-regulated KGF transgenic mice. Remarkably, we have found that ectopic KGF expression resulted in the emergence of hepatocytes within the islets of Langerhans in the pancreas. Additionally, significant intra-islet duct cell proliferation in the pancreata of transgenic KGF mice was observed. The unexpected appearance of hepatocytes and proliferation of intra-islet duct cells in the pancreata of these mice evidently stemmed directly from local exposure to KGF.",
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AU - Lee, Jae

AU - Arnush, Marc

AU - Gu, Danling

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