Pancreas of coxsackievirus-infected dams and their challenged pups

A complex issue

Sona Sarmirova, Maria Borsanyiova, Brigita Benkoova, Michaela Pospisilova, Rajkumar Arumugam, Katarina Berakova, Pavol Gomolcak, N R Jayagopala Reddy, Shubhada Bopegamage

Research output: Contribution to journalArticle

Abstract

Enteroviral infections are frequent, often asymptomatic in humans and during gravidity. The present study is an extension of our previous investigations where we had shown pancreatitis in challenged pups of CVB4-E2-infected dams. Present investigation describes the effect of gestational infection with this virus on the pancreas of both dams and their challenged pups. Gravid CD1 outbred mice were orally infected with CVB4-E2 virus at different gestation times. Pups were challenged orally with the same virus after 25 days of birth. Organs were collected at selected intervals postinfection (p.i.), and replicating virus and viral-RNA copies were analyzed. Additional readouts included histopathology and immunohistochemical (IHC) analysis for localization and identification of Ly6G+ cells (neutrophils), CD11b+ cells (macrophages), and viral protein in pancreatic tissue sections of the infected dams and their challenged pups. Our results show the presence of replicating virus in the pancreas of infected dams and their challenged pups, with inflammation leading to chronic necrotizing pancreatitis and atrophy of pancreatic acini of the dams and their offspring. IHC analysis of the infiltrating cells showed pronounced Ly6G+ neutrophils in dams only, whereas CD11b+ macrophages were present in tissues of both, the pups and the dams. Time of infection during gravidity as well as the p.i. intervals when mice were sacrificed influenced the pancreatic pathophysiology in both groups. We conclude that coxsackievirus infection during pregnancy is a risk factor for chronic affliction of the exocrine tissue and could affect endocrine pancreas in the mother and child.

Original languageEnglish (US)
Pages (from-to)207-221
Number of pages15
JournalVirulence
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Enterovirus
Pancreas
Gravidity
Viruses
Neutrophils
Macrophages
Coxsackievirus Infections
Pregnancy
Chronic Pancreatitis
Viral RNA
Viral Proteins
Virus Diseases
Infection
Islets of Langerhans
Pancreatitis
Atrophy
Mothers
Parturition
Inflammation

Keywords

  • Coxsackievirus B4-E2
  • dams
  • gravidity
  • mice
  • offspring
  • pancreas

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Sarmirova, S., Borsanyiova, M., Benkoova, B., Pospisilova, M., Arumugam, R., Berakova, K., ... Bopegamage, S. (2019). Pancreas of coxsackievirus-infected dams and their challenged pups: A complex issue. Virulence, 10(1), 207-221. https://doi.org/10.1080/21505594.2019.1589364

Pancreas of coxsackievirus-infected dams and their challenged pups : A complex issue. / Sarmirova, Sona; Borsanyiova, Maria; Benkoova, Brigita; Pospisilova, Michaela; Arumugam, Rajkumar; Berakova, Katarina; Gomolcak, Pavol; Reddy, N R Jayagopala; Bopegamage, Shubhada.

In: Virulence, Vol. 10, No. 1, 01.01.2019, p. 207-221.

Research output: Contribution to journalArticle

Sarmirova, S, Borsanyiova, M, Benkoova, B, Pospisilova, M, Arumugam, R, Berakova, K, Gomolcak, P, Reddy, NRJ & Bopegamage, S 2019, 'Pancreas of coxsackievirus-infected dams and their challenged pups: A complex issue', Virulence, vol. 10, no. 1, pp. 207-221. https://doi.org/10.1080/21505594.2019.1589364
Sarmirova S, Borsanyiova M, Benkoova B, Pospisilova M, Arumugam R, Berakova K et al. Pancreas of coxsackievirus-infected dams and their challenged pups: A complex issue. Virulence. 2019 Jan 1;10(1):207-221. https://doi.org/10.1080/21505594.2019.1589364
Sarmirova, Sona ; Borsanyiova, Maria ; Benkoova, Brigita ; Pospisilova, Michaela ; Arumugam, Rajkumar ; Berakova, Katarina ; Gomolcak, Pavol ; Reddy, N R Jayagopala ; Bopegamage, Shubhada. / Pancreas of coxsackievirus-infected dams and their challenged pups : A complex issue. In: Virulence. 2019 ; Vol. 10, No. 1. pp. 207-221.
@article{8c3e8e050ab04071adc619bd947c9d55,
title = "Pancreas of coxsackievirus-infected dams and their challenged pups: A complex issue",
abstract = "Enteroviral infections are frequent, often asymptomatic in humans and during gravidity. The present study is an extension of our previous investigations where we had shown pancreatitis in challenged pups of CVB4-E2-infected dams. Present investigation describes the effect of gestational infection with this virus on the pancreas of both dams and their challenged pups. Gravid CD1 outbred mice were orally infected with CVB4-E2 virus at different gestation times. Pups were challenged orally with the same virus after 25 days of birth. Organs were collected at selected intervals postinfection (p.i.), and replicating virus and viral-RNA copies were analyzed. Additional readouts included histopathology and immunohistochemical (IHC) analysis for localization and identification of Ly6G+ cells (neutrophils), CD11b+ cells (macrophages), and viral protein in pancreatic tissue sections of the infected dams and their challenged pups. Our results show the presence of replicating virus in the pancreas of infected dams and their challenged pups, with inflammation leading to chronic necrotizing pancreatitis and atrophy of pancreatic acini of the dams and their offspring. IHC analysis of the infiltrating cells showed pronounced Ly6G+ neutrophils in dams only, whereas CD11b+ macrophages were present in tissues of both, the pups and the dams. Time of infection during gravidity as well as the p.i. intervals when mice were sacrificed influenced the pancreatic pathophysiology in both groups. We conclude that coxsackievirus infection during pregnancy is a risk factor for chronic affliction of the exocrine tissue and could affect endocrine pancreas in the mother and child.",
keywords = "Coxsackievirus B4-E2, dams, gravidity, mice, offspring, pancreas",
author = "Sona Sarmirova and Maria Borsanyiova and Brigita Benkoova and Michaela Pospisilova and Rajkumar Arumugam and Katarina Berakova and Pavol Gomolcak and Reddy, {N R Jayagopala} and Shubhada Bopegamage",
year = "2019",
month = "1",
day = "1",
doi = "10.1080/21505594.2019.1589364",
language = "English (US)",
volume = "10",
pages = "207--221",
journal = "Virulence",
issn = "2150-5594",
publisher = "Landes Bioscience",
number = "1",

}

TY - JOUR

T1 - Pancreas of coxsackievirus-infected dams and their challenged pups

T2 - A complex issue

AU - Sarmirova, Sona

AU - Borsanyiova, Maria

AU - Benkoova, Brigita

AU - Pospisilova, Michaela

AU - Arumugam, Rajkumar

AU - Berakova, Katarina

AU - Gomolcak, Pavol

AU - Reddy, N R Jayagopala

AU - Bopegamage, Shubhada

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Enteroviral infections are frequent, often asymptomatic in humans and during gravidity. The present study is an extension of our previous investigations where we had shown pancreatitis in challenged pups of CVB4-E2-infected dams. Present investigation describes the effect of gestational infection with this virus on the pancreas of both dams and their challenged pups. Gravid CD1 outbred mice were orally infected with CVB4-E2 virus at different gestation times. Pups were challenged orally with the same virus after 25 days of birth. Organs were collected at selected intervals postinfection (p.i.), and replicating virus and viral-RNA copies were analyzed. Additional readouts included histopathology and immunohistochemical (IHC) analysis for localization and identification of Ly6G+ cells (neutrophils), CD11b+ cells (macrophages), and viral protein in pancreatic tissue sections of the infected dams and their challenged pups. Our results show the presence of replicating virus in the pancreas of infected dams and their challenged pups, with inflammation leading to chronic necrotizing pancreatitis and atrophy of pancreatic acini of the dams and their offspring. IHC analysis of the infiltrating cells showed pronounced Ly6G+ neutrophils in dams only, whereas CD11b+ macrophages were present in tissues of both, the pups and the dams. Time of infection during gravidity as well as the p.i. intervals when mice were sacrificed influenced the pancreatic pathophysiology in both groups. We conclude that coxsackievirus infection during pregnancy is a risk factor for chronic affliction of the exocrine tissue and could affect endocrine pancreas in the mother and child.

AB - Enteroviral infections are frequent, often asymptomatic in humans and during gravidity. The present study is an extension of our previous investigations where we had shown pancreatitis in challenged pups of CVB4-E2-infected dams. Present investigation describes the effect of gestational infection with this virus on the pancreas of both dams and their challenged pups. Gravid CD1 outbred mice were orally infected with CVB4-E2 virus at different gestation times. Pups were challenged orally with the same virus after 25 days of birth. Organs were collected at selected intervals postinfection (p.i.), and replicating virus and viral-RNA copies were analyzed. Additional readouts included histopathology and immunohistochemical (IHC) analysis for localization and identification of Ly6G+ cells (neutrophils), CD11b+ cells (macrophages), and viral protein in pancreatic tissue sections of the infected dams and their challenged pups. Our results show the presence of replicating virus in the pancreas of infected dams and their challenged pups, with inflammation leading to chronic necrotizing pancreatitis and atrophy of pancreatic acini of the dams and their offspring. IHC analysis of the infiltrating cells showed pronounced Ly6G+ neutrophils in dams only, whereas CD11b+ macrophages were present in tissues of both, the pups and the dams. Time of infection during gravidity as well as the p.i. intervals when mice were sacrificed influenced the pancreatic pathophysiology in both groups. We conclude that coxsackievirus infection during pregnancy is a risk factor for chronic affliction of the exocrine tissue and could affect endocrine pancreas in the mother and child.

KW - Coxsackievirus B4-E2

KW - dams

KW - gravidity

KW - mice

KW - offspring

KW - pancreas

UR - http://www.scopus.com/inward/record.url?scp=85063611022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063611022&partnerID=8YFLogxK

U2 - 10.1080/21505594.2019.1589364

DO - 10.1080/21505594.2019.1589364

M3 - Article

VL - 10

SP - 207

EP - 221

JO - Virulence

JF - Virulence

SN - 2150-5594

IS - 1

ER -