Paclitaxel-loaded biodegradable nanoparticles developed by direct dialysis and electrodydrodynamic atomization methods

Jingwei Xie, Chi Hwa Wang

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

This paper presents a new method for fabrication of biodegradable polymeric nanoparticles as carriers for anticancer drug paclitaxel. Emulsion solvent evaporation method was frequently employed to prepare polymeric nanoparticles for drug delivery systems in which the emulsifier such as PVA has to be used. In this study, direct dialysis was employed to fabricate paclitaxel-loaded biodegradable polymeric nanoparticles thus avoiding using emulsifier which potentially could remain on the surface of the nanoparticles and affect the biodegradability, biodistribution and drug-release behavior. The nanoparticles were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM) for their morphology; laser scattering particle size analyzer for particle size distribution; and, zeta potential analyzer for their surface charge. The physical status of paclitaxel in polymer matrix by different theory drug loadings was studied by differential scanning calorimetry (DSC) and X-ray diffractometry pattern. The encapsulation efficiency and in vitro release profile were measured by high performance liquid chromatography (HPLC). C6 glioma cell line was used to evaluate the particle cellular uptake and cytotoxicity.

Original languageEnglish (US)
Pages2193-2202
Number of pages10
StatePublished - Dec 1 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Conference

Conference2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

Fingerprint

Dialysis
Atomization
Nanoparticles
Biodegradability
High performance liquid chromatography
Zeta potential
Cytotoxicity
Surface charge
Polymer matrix
Encapsulation
Particle size analysis
X ray diffraction analysis
Emulsions
Differential scanning calorimetry
Atomic force microscopy
Evaporation
Particle size
Cells
Scattering
Fabrication

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Xie, J., & Wang, C. H. (2004). Paclitaxel-loaded biodegradable nanoparticles developed by direct dialysis and electrodydrodynamic atomization methods. 2193-2202. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.

Paclitaxel-loaded biodegradable nanoparticles developed by direct dialysis and electrodydrodynamic atomization methods. / Xie, Jingwei; Wang, Chi Hwa.

2004. 2193-2202 Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.

Research output: Contribution to conferencePaper

Xie, J & Wang, CH 2004, 'Paclitaxel-loaded biodegradable nanoparticles developed by direct dialysis and electrodydrodynamic atomization methods' Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States, 11/7/04 - 11/12/04, pp. 2193-2202.
Xie J, Wang CH. Paclitaxel-loaded biodegradable nanoparticles developed by direct dialysis and electrodydrodynamic atomization methods. 2004. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.
Xie, Jingwei ; Wang, Chi Hwa. / Paclitaxel-loaded biodegradable nanoparticles developed by direct dialysis and electrodydrodynamic atomization methods. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.10 p.
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