p24 and p26, structurally related cell surface molecules identified by monoclonal antibody BA-2

Tucker W. LeBlen, Samuel J. Pirruccello, Robert T. McCormack, J. Garrett Bradley

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This paper describes additional structural analyses of the p24 cell surface molecule recognized by monoclonal antibody BA-2. Since BA-2 is broadly reactive with a variety of normal and malignant lymphohematopoietic and nonlymphohematopoietic cells, we examined the structure of p24 expressed on different cell types. Tryptic peptide mapping and 2-dimensional gel electrophoresis of p24 isolated from colon carcinoma cells, fresh leukemic cells, leukemic cell lines, and activated T-cells indicated that p24 exhibits no structural polymorphism within the cells examined. As has recently been demonstrated with several other cell surface molecules, p24 is shown 10 possess a covalently-attached fatty acid, based on the incorporation of [3H]palmitate. We have also identified an additional protein, designated p26, that is coprecipitated with p24. The p26 molecule is not disulfide-linked to p24, and can be immunoprecipitated from a variety of 125I- or [35S]methionine-labeled cells. V8 protease peptide mapping indicated that p24 and p26 are structurally homologous. Pulse-chase analysis using [35S]methionine and digestion with endoglycosidase-F indicated that p24 and p26 are probably derived from a p23 precursor, but no precursor-product relationship exists between p24 and p26. Based on this data we propose that p24 and p26 are most likely differentially-processed protein products of the same gene.

Original languageEnglish (US)
Pages (from-to)1185-1194
Number of pages10
JournalMolecular Immunology
Volume22
Issue number10
DOIs
StatePublished - Oct 1985

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Monoclonal Antibodies
Peptide Mapping
Methionine
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
N-methyl-valyl-amiclenomycin
Palmitates
Disulfides
Electrophoresis
Digestion
Colon
Proteins
Fatty Acids
Gels
Carcinoma
T-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

Cite this

p24 and p26, structurally related cell surface molecules identified by monoclonal antibody BA-2. / LeBlen, Tucker W.; Pirruccello, Samuel J.; McCormack, Robert T.; Bradley, J. Garrett.

In: Molecular Immunology, Vol. 22, No. 10, 10.1985, p. 1185-1194.

Research output: Contribution to journalArticle

LeBlen, Tucker W. ; Pirruccello, Samuel J. ; McCormack, Robert T. ; Bradley, J. Garrett. / p24 and p26, structurally related cell surface molecules identified by monoclonal antibody BA-2. In: Molecular Immunology. 1985 ; Vol. 22, No. 10. pp. 1185-1194.
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