Ozonolysis of neem oil: Preparation and characterization of potent antibacterial agents against multidrug resistant bacterial strains

Paola De Oliveira, Nathália De Almeida, Martin Conda Sheridan, Rafael Do Prado Apparecido, Ana Camila Micheletti, Nadia Cristina Carvalho, Edson Dos Anjos Dos Santos, Maria Rita Marques, Eduardo De Arruda, Glaucia Braz Alcantara, Lincoln Carlos De Oliveira, Dênis De Lima, Adilson Beatriz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Neem, Azadirachta indica A. Juss, is endowed with relevant biological properties and its oil contains unsaturated fatty acids that are susceptible to structural modification by oxidative processes such as ozonolysis to form peroxides. Therefore, the aim of this work was the synthesis, physicochemical characterization, study of thermal behavior, and evaluation of the antimicrobial potential of neem ozonated oils. The ozonolysis reaction was performed over different periods of time, in the presence or absence of water at an ozone concentration of 63 mg L-1 O3/O2. The samples were characterized by 1H and 13C NMR spectroscopy, acid and iodine values and, DSC and TG/DTG thermal analyses. Additionally, quantitative 1H NMR spectroscopy was a very successful and useful tool to determine the unsaturation degree of samples. The products showed excellent broad-spectrum antimicrobial activity in comparison to other ozonated oils reported in the literature, with an MIC of <0.5 mg mL-1 for standard E. faecalis and clinical vancomycin resistant E. faecium, 5.0 mg mL-1 for clinical multiresistant K. pneumoniae (KPC), 2 mg mL-1 for standard S. aureus, and 3 mg mL-1 for methicillin-resistant S. aureus (MRSA). This is the first report on the antimicrobial action of neem oil after the ozonation process. The ozonated neem oils were investigated for their cytotoxicity against two normal human cell lines (HaCaT and HCEC). And the results show the products possess low toxicity. Our studies suggest the compounds can find potential application in the treatment of chronic wounds and skin infections.

Original languageEnglish (US)
Pages (from-to)34356-34365
Number of pages10
JournalRSC Advances
Volume7
Issue number55
DOIs
StatePublished - Jan 1 2017

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Bactericides
Anti-Bacterial Agents
Nuclear magnetic resonance spectroscopy
Oils
Ozonization
Methicillin
Ozone
Peroxides
Vancomycin
Cytotoxicity
Unsaturated Fatty Acids
Iodine
Toxicity
Skin
Unsaturated fatty acids
Cells
Acids
Water
neem oil
Hot Temperature

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

De Oliveira, P., De Almeida, N., Conda Sheridan, M., Apparecido, R. D. P., Micheletti, A. C., Carvalho, N. C., ... Beatriz, A. (2017). Ozonolysis of neem oil: Preparation and characterization of potent antibacterial agents against multidrug resistant bacterial strains. RSC Advances, 7(55), 34356-34365. https://doi.org/10.1039/c7ra00574a

Ozonolysis of neem oil : Preparation and characterization of potent antibacterial agents against multidrug resistant bacterial strains. / De Oliveira, Paola; De Almeida, Nathália; Conda Sheridan, Martin; Apparecido, Rafael Do Prado; Micheletti, Ana Camila; Carvalho, Nadia Cristina; Dos Santos, Edson Dos Anjos; Marques, Maria Rita; De Arruda, Eduardo; Alcantara, Glaucia Braz; De Oliveira, Lincoln Carlos; De Lima, Dênis; Beatriz, Adilson.

In: RSC Advances, Vol. 7, No. 55, 01.01.2017, p. 34356-34365.

Research output: Contribution to journalArticle

De Oliveira, P, De Almeida, N, Conda Sheridan, M, Apparecido, RDP, Micheletti, AC, Carvalho, NC, Dos Santos, EDA, Marques, MR, De Arruda, E, Alcantara, GB, De Oliveira, LC, De Lima, D & Beatriz, A 2017, 'Ozonolysis of neem oil: Preparation and characterization of potent antibacterial agents against multidrug resistant bacterial strains', RSC Advances, vol. 7, no. 55, pp. 34356-34365. https://doi.org/10.1039/c7ra00574a
De Oliveira, Paola ; De Almeida, Nathália ; Conda Sheridan, Martin ; Apparecido, Rafael Do Prado ; Micheletti, Ana Camila ; Carvalho, Nadia Cristina ; Dos Santos, Edson Dos Anjos ; Marques, Maria Rita ; De Arruda, Eduardo ; Alcantara, Glaucia Braz ; De Oliveira, Lincoln Carlos ; De Lima, Dênis ; Beatriz, Adilson. / Ozonolysis of neem oil : Preparation and characterization of potent antibacterial agents against multidrug resistant bacterial strains. In: RSC Advances. 2017 ; Vol. 7, No. 55. pp. 34356-34365.
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AU - Micheletti, Ana Camila

AU - Carvalho, Nadia Cristina

AU - Dos Santos, Edson Dos Anjos

AU - Marques, Maria Rita

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AU - De Oliveira, Lincoln Carlos

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