Oxygen reactivity of PutA from Helicobacter species and proline-linked oxidative stress

Navasona Krishnan, Donald F Becker

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Proline is converted to glutamate in two successive steps by the proline utilization A (PutA) flavoenzyme in gram-negative bacteria. PutA contains a proline dehydrogenase domain that catalyzes the flavin adenine dinucleotide (FAD)-dependent oxidation of proline to Δ1-pyrroline-5- carboxylate (P5C) and a P5C dehydrogenase domain that catalyzes the NAD +-dependent oxidation of P5C to glutamate. Here, we characterize PutA from Helicobacter hepaticus (PutAHh) and Helicobacter pylori (PutAHp) to provide new insights into proline metabolism in these gastrointestinal pathogens. Both PutAHh and PutAHp lack DNA binding activity, in contrast to PutA from Escherichia coli (PutA Ec), which both regulates and catalyzes proline utilization. PutAHh and PutAHp display catalytic activities similar to that of PutAEc but have higher oxygen reactivity. PutAHh and PutAHp exhibit 100-fold-higher turnover numbers (∼30 min -1) than PutAEc (<0.3 min-1) using oxygen as an electron acceptor during catalytic turnover with proline. Consistent with increased oxygen reactivity, PutAHh forms a reversible FAD-sulfite adduct. The significance of increased oxygen reactivity in PutAHh and PutAHp was probed by oxidative stress studies in E. coli. Expression of PutAEc and PutA from Bradyrhizobium japonicum, which exhibit low oxygen reactivity, does not diminish stress survival rates of E. coli cell cultures. In contrast, PutAHp and PutAHh expression dramatically reduces E. coli cell survival and is correlated with relatively lower proline levels and increased hydrogen peroxide formation. The discovery of reduced oxygen species formation by PutA suggests that proline catabolism may influence redox homeostasis in the ecological niches of these Helicobacter species.

Original languageEnglish (US)
Pages (from-to)1227-1235
Number of pages9
JournalJournal of bacteriology
Volume188
Issue number4
DOIs
StatePublished - Feb 1 2006

Fingerprint

Helicobacter
Proline
Helicobacter hepaticus
Oxidative Stress
Oxygen
Helicobacter pylori
Escherichia coli
Flavin-Adenine Dinucleotide
Glutamic Acid
Proline Oxidase
Bradyrhizobium
Sulfites
Gram-Negative Bacteria
NAD
Hydrogen Peroxide
Oxidation-Reduction

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Oxygen reactivity of PutA from Helicobacter species and proline-linked oxidative stress. / Krishnan, Navasona; Becker, Donald F.

In: Journal of bacteriology, Vol. 188, No. 4, 01.02.2006, p. 1227-1235.

Research output: Contribution to journalArticle

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