Oxaliplatin induces hyperexcitability at motor and autonomic neuromuscular junctions through effects on voltage-gated sodium channels

Richard G. Webster, Keith L. Brain, Richard H. Wilson, Jean L. Grem, Angela Vincent

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

1 Oxaliplatin, an effective cytotoxic treatment in combination with 5-fluorouracil for colorectal cancer, is associated with sensory, motor and autonomic neurotoxicity. Motor symptoms include hyperexcitability while autonomic effects include urinary retention, but the cause of these side-effects is unknown. We examined the effects on motor nerve function in the mouse hemidiaphragm and on the autonomic system in the vas deferens. 2 In the mouse diaphragm, oxaliplatin (0.5 mM) induced multiple endplate potentials (EPPs) following a single stimulus, and was associated with an increase in spontaneous miniature EPP frequency. In the vas deferens, spontaneous excitatory junction potential frequency was increased after 30 min exposure to oxaliplatin; no changes in resting Ca 2+ concentration in nerve terminal varicosities were observed, and recovery after stimuli trains was unaffected. 3 In both tissues, an oxaliplatin-induced increase in spontaneous activity was prevented by the voltage-gated Na + channel blocker tetrodotoxin (TTX). Carbamazepine (0.3 mM) also prevented multiple EPPs and the increase in spontaneous activity in both tissues. In diaphragm, β-pompilidotoxin (100 μM), which slows Na + channel inactivation, induced multiple EPPs similar to oxaliplatin's effect. By contrast, blockers of K + channels (4-aminopyridine and apamin) did not replicate oxaliplatin-induced hyperexcitability in the diaphragm. 4 The prevention of hyperexcitability by TTX blockade implies that oxaliplatin acts on nerve conduction rather than by effecting repolarisation. The similarity between β-pompilidotoxin and oxaliplatin suggests that alteration of voltage-gated Na + channel kinetics is likely to underlie the acute neurotoxic actions of oxaliplatin.

Original languageEnglish (US)
Pages (from-to)1027-1039
Number of pages13
JournalBritish Journal of Pharmacology
Volume146
Issue number7
DOIs
StatePublished - Dec 1 2005

Fingerprint

oxaliplatin
Voltage-Gated Sodium Channels
Neuromuscular Junction
Diaphragm
Vas Deferens
Tetrodotoxin
Autonomic Agents
Apamin
4-Aminopyridine
Urinary Retention
Neural Conduction
Carbamazepine

Keywords

  • Carbamazepine
  • Hyperexcitability
  • Neuromuscular junction
  • Neuromyotonia
  • Neurotoxicity
  • Oxaliplatin
  • Voltage-activated Na channels
  • β-pompilidotoxin

ASJC Scopus subject areas

  • Pharmacology

Cite this

Oxaliplatin induces hyperexcitability at motor and autonomic neuromuscular junctions through effects on voltage-gated sodium channels. / Webster, Richard G.; Brain, Keith L.; Wilson, Richard H.; Grem, Jean L.; Vincent, Angela.

In: British Journal of Pharmacology, Vol. 146, No. 7, 01.12.2005, p. 1027-1039.

Research output: Contribution to journalArticle

Webster, Richard G. ; Brain, Keith L. ; Wilson, Richard H. ; Grem, Jean L. ; Vincent, Angela. / Oxaliplatin induces hyperexcitability at motor and autonomic neuromuscular junctions through effects on voltage-gated sodium channels. In: British Journal of Pharmacology. 2005 ; Vol. 146, No. 7. pp. 1027-1039.
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abstract = "1 Oxaliplatin, an effective cytotoxic treatment in combination with 5-fluorouracil for colorectal cancer, is associated with sensory, motor and autonomic neurotoxicity. Motor symptoms include hyperexcitability while autonomic effects include urinary retention, but the cause of these side-effects is unknown. We examined the effects on motor nerve function in the mouse hemidiaphragm and on the autonomic system in the vas deferens. 2 In the mouse diaphragm, oxaliplatin (0.5 mM) induced multiple endplate potentials (EPPs) following a single stimulus, and was associated with an increase in spontaneous miniature EPP frequency. In the vas deferens, spontaneous excitatory junction potential frequency was increased after 30 min exposure to oxaliplatin; no changes in resting Ca 2+ concentration in nerve terminal varicosities were observed, and recovery after stimuli trains was unaffected. 3 In both tissues, an oxaliplatin-induced increase in spontaneous activity was prevented by the voltage-gated Na + channel blocker tetrodotoxin (TTX). Carbamazepine (0.3 mM) also prevented multiple EPPs and the increase in spontaneous activity in both tissues. In diaphragm, β-pompilidotoxin (100 μM), which slows Na + channel inactivation, induced multiple EPPs similar to oxaliplatin's effect. By contrast, blockers of K + channels (4-aminopyridine and apamin) did not replicate oxaliplatin-induced hyperexcitability in the diaphragm. 4 The prevention of hyperexcitability by TTX blockade implies that oxaliplatin acts on nerve conduction rather than by effecting repolarisation. The similarity between β-pompilidotoxin and oxaliplatin suggests that alteration of voltage-gated Na + channel kinetics is likely to underlie the acute neurotoxic actions of oxaliplatin.",
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