Overexpression of patatin-related phospholipase AIIIβ altered the content and composition of sphingolipids in Arabidopsis

Maoyin Li, Jonathan E Markham, Xuemin Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In plants, fatty acids are primarily synthesized in plastids and then transported to the endoplasmic reticulum (ER) for synthesis of most of the complex membrane lipids, including glycerolipids and sphingolipids. The first step of sphingolipid synthesis, which uses a fatty acid and a serine as substrates, is critical for sphingolipid homeostasis; its disruption leads to an altered plant growth. Phospholipase As have been implicated in the trafficking of fatty acids from plastids to the ER. Previously, we found that overexpression of a patatin-related phospholipase, pPLAIIIβ, resulted in a smaller plant size and altered anisotropic cell expansion. Here, we determined the content and composition of sphingolipids in pPLAIIIβ-knockout and overexpression plants (pPLAIIIβ-KO and -OE). 3-keto-sphinganine, the product of the first step of sphingolipid synthesis, had a 26% decrease in leaves of pPLAIIIβ-KO while a 52% increase in pPLAIIIβ-OE compared to wild type (WT). The levels of free long-chain base species, dihydroxy-C18:0 and trihydroxy-18:0 (d18:0 and t18:0), were 38 and 97% higher, respectively, in pPLAIIIβ-OE than in WT. The level of complex sphingolipids ceramide d18:0–16:0 and t18:1–16:0 had a twofold increase in pPLAIIIβ-OE. The level of hydroxy ceramide d18:0–h16:0 was 72% higher in pPLAIIIβ-OE compared to WT. The levels of several species of glucosylceramide and glycosylinositolphosphoceramide tended to be higher in pPLAIIIβ-OE than in WT. The total content of the complex sphingolipids showed a slightly higher in pPLAIIIβ-OE than in WT. These results revealed an involvement of phospholipase-mediated lipid homeostasis in plant growth.

Original languageEnglish (US)
Article number278
JournalFrontiers in Plant Science
Volume5
Issue numberOCT
DOIs
StatePublished - Oct 21 2014

Fingerprint

patatin
sphingolipids
Arabidopsis
ceramides
fatty acids
endoplasmic reticulum
plastids
synthesis
homeostasis
plant growth
lipids
phospholipases
serine

Keywords

  • Arabidopsis thaliana
  • Fatty acyl flux
  • Patatin-related phospholipase
  • Plant growth
  • Sphingolipid

ASJC Scopus subject areas

  • Plant Science

Cite this

Overexpression of patatin-related phospholipase AIIIβ altered the content and composition of sphingolipids in Arabidopsis. / Li, Maoyin; Markham, Jonathan E; Wang, Xuemin.

In: Frontiers in Plant Science, Vol. 5, No. OCT, 278, 21.10.2014.

Research output: Contribution to journalArticle

@article{a278200e1bea431ca06dc2d005cdceb7,
title = "Overexpression of patatin-related phospholipase AIIIβ altered the content and composition of sphingolipids in Arabidopsis",
abstract = "In plants, fatty acids are primarily synthesized in plastids and then transported to the endoplasmic reticulum (ER) for synthesis of most of the complex membrane lipids, including glycerolipids and sphingolipids. The first step of sphingolipid synthesis, which uses a fatty acid and a serine as substrates, is critical for sphingolipid homeostasis; its disruption leads to an altered plant growth. Phospholipase As have been implicated in the trafficking of fatty acids from plastids to the ER. Previously, we found that overexpression of a patatin-related phospholipase, pPLAIIIβ, resulted in a smaller plant size and altered anisotropic cell expansion. Here, we determined the content and composition of sphingolipids in pPLAIIIβ-knockout and overexpression plants (pPLAIIIβ-KO and -OE). 3-keto-sphinganine, the product of the first step of sphingolipid synthesis, had a 26{\%} decrease in leaves of pPLAIIIβ-KO while a 52{\%} increase in pPLAIIIβ-OE compared to wild type (WT). The levels of free long-chain base species, dihydroxy-C18:0 and trihydroxy-18:0 (d18:0 and t18:0), were 38 and 97{\%} higher, respectively, in pPLAIIIβ-OE than in WT. The level of complex sphingolipids ceramide d18:0–16:0 and t18:1–16:0 had a twofold increase in pPLAIIIβ-OE. The level of hydroxy ceramide d18:0–h16:0 was 72{\%} higher in pPLAIIIβ-OE compared to WT. The levels of several species of glucosylceramide and glycosylinositolphosphoceramide tended to be higher in pPLAIIIβ-OE than in WT. The total content of the complex sphingolipids showed a slightly higher in pPLAIIIβ-OE than in WT. These results revealed an involvement of phospholipase-mediated lipid homeostasis in plant growth.",
keywords = "Arabidopsis thaliana, Fatty acyl flux, Patatin-related phospholipase, Plant growth, Sphingolipid",
author = "Maoyin Li and Markham, {Jonathan E} and Xuemin Wang",
year = "2014",
month = "10",
day = "21",
doi = "10.3389/fpls.2014.00553",
language = "English (US)",
volume = "5",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S. A.",
number = "OCT",

}

TY - JOUR

T1 - Overexpression of patatin-related phospholipase AIIIβ altered the content and composition of sphingolipids in Arabidopsis

AU - Li, Maoyin

AU - Markham, Jonathan E

AU - Wang, Xuemin

PY - 2014/10/21

Y1 - 2014/10/21

N2 - In plants, fatty acids are primarily synthesized in plastids and then transported to the endoplasmic reticulum (ER) for synthesis of most of the complex membrane lipids, including glycerolipids and sphingolipids. The first step of sphingolipid synthesis, which uses a fatty acid and a serine as substrates, is critical for sphingolipid homeostasis; its disruption leads to an altered plant growth. Phospholipase As have been implicated in the trafficking of fatty acids from plastids to the ER. Previously, we found that overexpression of a patatin-related phospholipase, pPLAIIIβ, resulted in a smaller plant size and altered anisotropic cell expansion. Here, we determined the content and composition of sphingolipids in pPLAIIIβ-knockout and overexpression plants (pPLAIIIβ-KO and -OE). 3-keto-sphinganine, the product of the first step of sphingolipid synthesis, had a 26% decrease in leaves of pPLAIIIβ-KO while a 52% increase in pPLAIIIβ-OE compared to wild type (WT). The levels of free long-chain base species, dihydroxy-C18:0 and trihydroxy-18:0 (d18:0 and t18:0), were 38 and 97% higher, respectively, in pPLAIIIβ-OE than in WT. The level of complex sphingolipids ceramide d18:0–16:0 and t18:1–16:0 had a twofold increase in pPLAIIIβ-OE. The level of hydroxy ceramide d18:0–h16:0 was 72% higher in pPLAIIIβ-OE compared to WT. The levels of several species of glucosylceramide and glycosylinositolphosphoceramide tended to be higher in pPLAIIIβ-OE than in WT. The total content of the complex sphingolipids showed a slightly higher in pPLAIIIβ-OE than in WT. These results revealed an involvement of phospholipase-mediated lipid homeostasis in plant growth.

AB - In plants, fatty acids are primarily synthesized in plastids and then transported to the endoplasmic reticulum (ER) for synthesis of most of the complex membrane lipids, including glycerolipids and sphingolipids. The first step of sphingolipid synthesis, which uses a fatty acid and a serine as substrates, is critical for sphingolipid homeostasis; its disruption leads to an altered plant growth. Phospholipase As have been implicated in the trafficking of fatty acids from plastids to the ER. Previously, we found that overexpression of a patatin-related phospholipase, pPLAIIIβ, resulted in a smaller plant size and altered anisotropic cell expansion. Here, we determined the content and composition of sphingolipids in pPLAIIIβ-knockout and overexpression plants (pPLAIIIβ-KO and -OE). 3-keto-sphinganine, the product of the first step of sphingolipid synthesis, had a 26% decrease in leaves of pPLAIIIβ-KO while a 52% increase in pPLAIIIβ-OE compared to wild type (WT). The levels of free long-chain base species, dihydroxy-C18:0 and trihydroxy-18:0 (d18:0 and t18:0), were 38 and 97% higher, respectively, in pPLAIIIβ-OE than in WT. The level of complex sphingolipids ceramide d18:0–16:0 and t18:1–16:0 had a twofold increase in pPLAIIIβ-OE. The level of hydroxy ceramide d18:0–h16:0 was 72% higher in pPLAIIIβ-OE compared to WT. The levels of several species of glucosylceramide and glycosylinositolphosphoceramide tended to be higher in pPLAIIIβ-OE than in WT. The total content of the complex sphingolipids showed a slightly higher in pPLAIIIβ-OE than in WT. These results revealed an involvement of phospholipase-mediated lipid homeostasis in plant growth.

KW - Arabidopsis thaliana

KW - Fatty acyl flux

KW - Patatin-related phospholipase

KW - Plant growth

KW - Sphingolipid

UR - http://www.scopus.com/inward/record.url?scp=84908244196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908244196&partnerID=8YFLogxK

U2 - 10.3389/fpls.2014.00553

DO - 10.3389/fpls.2014.00553

M3 - Article

VL - 5

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

IS - OCT

M1 - 278

ER -