Overaccumulation of γ-glutamylcysteine in a jasmonate-hypersensitive arabidopsis mutant causes jasmonate-dependent growth inhibition

Hsin Ho Wei, Martha Rowe, Jean Jack M. Riethoven, Ryan Grove, Jiri Adamec, Yusuke Jikumaru, Paul Staswick

Research output: Contribution to journalArticle

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Abstract

Glutathione (GSH) is essential for many aspects of plant biology and is associated with jasmonate signaling in stress responses. We characterized an Arabidopsis (Arabidopsis thaliana) jasmonate-hypersensitive mutant (jah2) with seedling root growth 100-fold more sensitive to inhibition by the hormone jasmonyl-isoleucine than the wild type. Genetic mapping and genome sequencing determined that the mutation is in intron 6 of GLUTATHIONE SYNTHETASE2, encoding the enzyme that converts γ-glutamylcysteine (γ-EC) to GSH. The level of GSH in jah2 was 71% of the wild type, while the phytoalexin-deficient2-1 (pad2-1) mutant, defective in GSH1 and having only 27% of wild-type GSH level, was not jasmonate hypersensitive. Growth defects for jah2, but not pad2, were also seen in plants grown to maturity. Surprisingly, all phenotypes in the jah2 pad2-1 double mutant were weaker than in jah2. Quantification of g-EC indicated these defects result from hyperaccumulation of this GSH precursor by 294-and 65-fold in jah2 and the double mutant, respectively. γ-EC reportedly partially substitutes for loss of GSH, but growth inhibition seen here was likely not due to an excess of total glutathione plus g-EC because their sum in jah2 pad2-1 was only 16% greater than in the wild type. Further, the jah2 phenotypes were lost in a jasmonic acid biosynthesis mutant background, indicating the effect of γ-EC is mediated through jasmonate signaling and not as a direct result of perturbed redox status.

Original languageEnglish (US)
Pages (from-to)1371-1381
Number of pages11
JournalPlant physiology
Volume169
Issue number2
DOIs
StatePublished - Sep 2015

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Arabidopsis
growth retardation
phytoalexins
mutants
Growth
Glutathione
glutathione
Phenotype
phenotype
Isoleucine
Chromosome Mapping
plant biology
isoleucine
jasmonic acid
Seedlings
Introns
chromosome mapping
Oxidation-Reduction
introns
stress response

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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Overaccumulation of γ-glutamylcysteine in a jasmonate-hypersensitive arabidopsis mutant causes jasmonate-dependent growth inhibition. / Wei, Hsin Ho; Rowe, Martha; Riethoven, Jean Jack M.; Grove, Ryan; Adamec, Jiri; Jikumaru, Yusuke; Staswick, Paul.

In: Plant physiology, Vol. 169, No. 2, 09.2015, p. 1371-1381.

Research output: Contribution to journalArticle

Wei, Hsin Ho ; Rowe, Martha ; Riethoven, Jean Jack M. ; Grove, Ryan ; Adamec, Jiri ; Jikumaru, Yusuke ; Staswick, Paul. / Overaccumulation of γ-glutamylcysteine in a jasmonate-hypersensitive arabidopsis mutant causes jasmonate-dependent growth inhibition. In: Plant physiology. 2015 ; Vol. 169, No. 2. pp. 1371-1381.
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