Otoconin-90, the mammalian otoconial matrix protein, contains two domains of homology to secretory phospholipase A2

Yunxia Wang, Paul E. Kowalski, Isolde Thalmann, David M. Ornitz, Dixie L. Mager, Rudiger Thalmann

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The ability to sense orientation relative to gravity requires dense particles, called otoconia, which are localized in the vestibular macular organs. In mammals, otoconia are composed of proteins (otoconins) and calcium carbonate crystals in a calcite lattice. Little is known about the mechanisms that regulate otoconial biosynthesis. To begin to elucidate these mechanisms, we have partially sequenced and cloned the major protein component of murine otoconia, otoconin-90 (OC90). The amino acid sequence identified an orphan chimeric human cDNA. Because of its similarity to secretory phospholipase A2 (sPLA2), this gene was referred to as PLA2-like (PLA2L) and enabled the identification of human Oc90. Partial murine cDNA and genomic clones were isolated and shown to be specifically expressed in the developing mouse otocyst. The mature mouse OC90 is composed of 453 residues and contains two domains homologous to sPLA2. The cloning of Oc90 will allow an examination of the role of this protein in otoconial biosynthesis and in diseases that affect the vestibular system.

Original languageEnglish (US)
Pages (from-to)15345-15350
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number26
DOIs
StatePublished - Dec 22 1998

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Secretory Phospholipase A2
Otolithic Membrane
Calcium Carbonate
Vestibular Diseases
Complementary DNA
Forensic Anthropology
Proteins
Orphaned Children
Gravitation
Organism Cloning
Mammals
Amino Acid Sequence
Clone Cells
Genes

ASJC Scopus subject areas

  • General

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Otoconin-90, the mammalian otoconial matrix protein, contains two domains of homology to secretory phospholipase A2. / Wang, Yunxia; Kowalski, Paul E.; Thalmann, Isolde; Ornitz, David M.; Mager, Dixie L.; Thalmann, Rudiger.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 26, 22.12.1998, p. 15345-15350.

Research output: Contribution to journalArticle

Wang, Yunxia ; Kowalski, Paul E. ; Thalmann, Isolde ; Ornitz, David M. ; Mager, Dixie L. ; Thalmann, Rudiger. / Otoconin-90, the mammalian otoconial matrix protein, contains two domains of homology to secretory phospholipase A2. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 26. pp. 15345-15350.
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AU - Thalmann, Rudiger

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