Otoconin-90 deletion leads to imbalance but normal hearing: A comparison with other otoconia mutants

X. Zhao, Sherri M Jones, E. N. Yamoah, Yunxia W Lundberg

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Our sense of gravitation and linear acceleration is mediated by stimulation of vestibular hair cells through displacement of otoconia in the utricle and saccule (the gravity receptor organ). We recently showed that otoconin-90 (Oc90) deletion led to formation of giant otoconia. In the present study, we determined the extent to which the giant otoconia affected balance and gravity receptor sensory input and compared the findings with other otoconia mutants. We employed a wide spectrum of balance behavioral tests, including reaching and air-righting reflexes, gait, swimming, beam-crossing, rotorod latencies, and a direct measure of gravity receptor input, vestibular evoked potentials (VsEPs). All tests on homozygous adult mutants consistently ranked the order of imbalance as (from worst to best) Nox3het<otopetrin 1tlt<Oc90 null<Oc90 wild type and C57Bl/6 mice using systematic statistical comparisons of the frequency of occurrence or the severity of abnormal functions. This order coincides with the degree of otoconia deficiencies and is consistent with VsEP measures. Notably, all mice (except Nox3het) showed remarkable learned adaptation to peripheral vestibular deficits by staying on the rotating rod significantly longer in each successive trial, and the rate and extent of such learned improvements ranked the same order as their initial balance ability. Despite the vestibular morbidity, Oc90 null mice had normal hearing, as measured by auditory brainstem responses (ABRs) and distortion products of otoacoustic emissions (DPOAEs). The study demonstrates that the remnant otoconia mass in Oc90 nulls does stimulate the gravity receptor organs, which was likely responsible for the improved balance performance relative to strains with absent otoconia. Furthermore, the combination of direct electrophysiological measures and a series of behavioral tests can be used to interpret the imbalance severity arising from altered inputs from the gravity receptor end organ.

Original languageEnglish (US)
Pages (from-to)289-299
Number of pages11
JournalNeuroscience
Volume153
Issue number1
DOIs
StatePublished - Apr 22 2008

Fingerprint

Otolithic Membrane
Hearing
Gravitation
Evoked Potentials
Altered Gravity
Vestibular Hair Cells
Righting Reflex
Saccule and Utricle
Aptitude
Brain Stem Auditory Evoked Potentials
Sensory Receptor Cells
Gait
Air
Morbidity

Keywords

  • adaptation
  • balance
  • compensation
  • hearing
  • otoconia
  • vestibule

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Otoconin-90 deletion leads to imbalance but normal hearing : A comparison with other otoconia mutants. / Zhao, X.; Jones, Sherri M; Yamoah, E. N.; Lundberg, Yunxia W.

In: Neuroscience, Vol. 153, No. 1, 22.04.2008, p. 289-299.

Research output: Contribution to journalArticle

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