Osteoblast adhesion on poly(L-lactic acid)/polystyrene demixed thin film blends: Effect of Nanotopography, surface chemistry, and wettability

Jung Yul Lim, Joshua C. Hansen, Christopher A. Siedlecki, Robert W. Hengstebeck, Juan Cheng, Nicholas Winogard, Henry J. Donahue

Research output: Contribution to journalArticle

116 Scopus citations


Biomaterial surface characteristics are critical cues that regulate cell function. We produced a novel series of poly(L-lactic acid) (PLLA) and polystyrene demixed nanotopographic films to provide nonbiological cell-stimulating cues. The increase in PLLA weight fraction (φ) in blend solutions resulted in topography changes in spin-cast films from pit-dominant to island-dominant morphologies having nanoscale depth or height (3-29 nm). Lower molecular weight PLLA segregated to the top surface of demixed films, as observed by X-ray photoelectron spectroscopy and secondary ion mass spectroscopy (SIMS). For φ ≥0.5, the topmost film layer was predominantly filled with PLLA (>96% by SIMS at 20-Å depth). Nanotextured substrata stimulated osteoblastic cell adhesion to a greater degree than did flat PLLA (φ = 1), and this effect was more pronounced for nanoisland (φ = 0.7 and 0.9) relative to nanopit topographies (φ = 0.5). Demixed films having relatively lower water contact angles generally enhanced cell adhesion and spreading. Our results reveal that cell adhesion is affected by surface chemistry, topography, and wettability simultaneously and that nanotextured surfaces may be utilized in regulating cell adhesion.

Original languageEnglish (US)
Pages (from-to)3319-3327
Number of pages9
Issue number6
StatePublished - Nov 1 2005


ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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