Oronasal and intramuscular vaccination of swine with a modified live porcine parvovirus vaccine: multiplication and transmission of the vaccine virus.

P. S. Paul, W. L. Mengeling

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Abstract

An attenuated strain NADL-2 of porcine parvovirus (PPV) has been used at the 54th cell culture passage as a modified live-virus (MLV) vaccine. The present study was conducted to determine the minimum immunizing dose of MLV, the extent of MLV multiplication in swine tissues, and its transmission from swine administered MLV oronasally or intramuscularly. Immune response to MLV was dose dependent and swine responded to as little as 10(2) median cell-culture infective doses (CCID50). A 10(5) CCID50 of MLV, the largest dose given, induced the best immune response and was used in subsequent experiments. Route of MLV administration also was found to be important. The MLV replicated in tissues of swine after IM inoculation; however, viral antigen in tissues was less, as measured by immunofluorescence, and serum hemagglutination-inhibition titers for PPV were lower in MLV-inoculated swine than we have previously observed in virulent PPV-inoculated swine. In contrast, oronasal inoculation with MLV did not consistently result in infection of pigs; only 5 of 23 swine had virologic and/or serologic evidence of infection. Virus transmission studies indicated that MLV is shed in feces, but shedding occurs later than that in virulent-PPV-inoculated swine and is inconsistent. Delayed transmission of MLV was observed in contact pigs, which were seronegative at 2 weeks, but became seropositive at 4 weeks--indicating that perhaps a virus population capable of infecting pigs by oronasal route was selected by passage through the pig.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)2481-2485
Number of pages5
JournalAmerican journal of veterinary research
Volume45
Issue number12
StatePublished - Dec 1 1984

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Porcine Parvovirus
Ungulate protoparvovirus 1
Vaccination
Swine
Vaccines
vaccination
vaccines
Viruses
viruses
swine
dosage
cell culture
immune response
Cell Culture Techniques
viral antigens
virus transmission
Viral Antigens
hemagglutination

ASJC Scopus subject areas

  • veterinary(all)

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Oronasal and intramuscular vaccination of swine with a modified live porcine parvovirus vaccine : multiplication and transmission of the vaccine virus. / Paul, P. S.; Mengeling, W. L.

In: American journal of veterinary research, Vol. 45, No. 12, 01.12.1984, p. 2481-2485.

Research output: Contribution to journalArticle

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