Ornithine decarboxylase encoded by chlorella virus PBCV-1

Tiara A. Morehead, James R. Gurnon, Byron Adams, Kenneth W. Nickerson, Lisa A. Fitzgerald, James L. Van Etten

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Sequence analysis of the 330-kb genome of chlorella virus PBCV-1 revealed an open reading frame, A207R, which encodes a protein with 37-41% amino acid identity to ornithine decarboxylase (ODC) from many eukaryotic organisms. The a207r gene was cloned and the protein was expressed as a His-A207R fusion protein in Escherichia coli. The recombinant protein catalyzes pyridoxal 5′-phosphate-dependent decarboxylation of ornithine to putrescine, the first step in the polyamine biosynthetic pathway. The enzyme has a pH optimum of 9.0 and a temperature optimum of 42°C, and it requires dithiothreitol for maximal activity. The enzyme has a Km for ornithine of 0.78 mM and a specific activity of 100 μmol/min/mg protein. PBCV-1 ODC is quite sensitive to the competitive inhibitor L-arginine and the irreversible inhibitor difluoromethylarginine but it is less sensitive to the irreversible inhibitor difluoromethylornithine. The a207r gene is expressed both early and late in PBCV-1 infection and is highly conserved among the chlorella viruses. The 42-kDa PBCV-1 ODC (372 amino acids) is the smallest ODC in the databases and, to our knowledge, is the first virus-encoded ODC.

Original languageEnglish (US)
Pages (from-to)165-175
Number of pages11
JournalVirology
Volume301
Issue number1
DOIs
StatePublished - Jan 1 2002

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Chlorella
Ornithine Decarboxylase
Viruses
Ornithine
Eflornithine
Amino Acids
Proteins
Decarboxylation
Pyridoxal Phosphate
Putrescine
Dithiothreitol
Escherichia coli Proteins
Biosynthetic Pathways
Polyamines
Enzymes
Recombinant Proteins
Open Reading Frames
Genes
Sequence Analysis
Arginine

Keywords

  • Chlorella viruses
  • Ornithine decarboxylase
  • Phycodnaviridae
  • Polyamines

ASJC Scopus subject areas

  • Virology

Cite this

Ornithine decarboxylase encoded by chlorella virus PBCV-1. / Morehead, Tiara A.; Gurnon, James R.; Adams, Byron; Nickerson, Kenneth W.; Fitzgerald, Lisa A.; Van Etten, James L.

In: Virology, Vol. 301, No. 1, 01.01.2002, p. 165-175.

Research output: Contribution to journalArticle

Morehead, TA, Gurnon, JR, Adams, B, Nickerson, KW, Fitzgerald, LA & Van Etten, JL 2002, 'Ornithine decarboxylase encoded by chlorella virus PBCV-1', Virology, vol. 301, no. 1, pp. 165-175. https://doi.org/10.1006/viro.2002.1573
Morehead, Tiara A. ; Gurnon, James R. ; Adams, Byron ; Nickerson, Kenneth W. ; Fitzgerald, Lisa A. ; Van Etten, James L. / Ornithine decarboxylase encoded by chlorella virus PBCV-1. In: Virology. 2002 ; Vol. 301, No. 1. pp. 165-175.
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