Ornithine decarboxylase and S adenosyl methionine decarboxylase in skin fibroblasts of normal and cystic fibrosis patients

Bruce Alan Buehler, R. Wright, S. Schott, B. Darby, O. M. Rennert

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The key enzymes in the synthesis of the naturally occurring polyamines, ornithine decarboxylase (ODC) and S-adenosyl methionine (SAM) decarboxylase, were investigated during cell growth and aging in fibroblast cultures from normal patients and patients with cystic fibrosis. A linear correlation between increased S-adenosyl methionine ine activity and putrescine concentration was apparent in all cell lines. A putrescine concentration of 0.8 mM was optimal for enhancement of SAM decarboxylase activity. The passage number of the cell line correlated inversely with maximal putrescine stimulated SAM decarboxylase activity, earlier passage numbers having the highest specific activity (Fig. 1). No significant differences in basal or putrescine stimulated SAM decarboxylase activity increased as the cell lines approached confluence. Activity was lowest during exponential growth (Fig. 3). ODC activity was increased during early exponential growth and fell as cells reached confluence (Fig. 4). No differences in ODC activity and putrescine inhibition between the normal and cystic fibrosis cell cultures at equivalent points of exponential growth were noted.

Original languageEnglish (US)
Pages (from-to)186-190
Number of pages5
JournalUnknown Journal
Volume11
Issue number3 (I)
DOIs
StatePublished - Jan 1 1977

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Ornithine Decarboxylase
Putrescine
Cystic Fibrosis
Fibroblasts
Skin
Growth
Cell Line
Cell Aging
Polyamines
Methionine
Cell Culture Techniques
methionine decarboxylase
Enzymes

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Ornithine decarboxylase and S adenosyl methionine decarboxylase in skin fibroblasts of normal and cystic fibrosis patients. / Buehler, Bruce Alan; Wright, R.; Schott, S.; Darby, B.; Rennert, O. M.

In: Unknown Journal, Vol. 11, No. 3 (I), 01.01.1977, p. 186-190.

Research output: Contribution to journalArticle

Buehler, Bruce Alan ; Wright, R. ; Schott, S. ; Darby, B. ; Rennert, O. M. / Ornithine decarboxylase and S adenosyl methionine decarboxylase in skin fibroblasts of normal and cystic fibrosis patients. In: Unknown Journal. 1977 ; Vol. 11, No. 3 (I). pp. 186-190.
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AU - Rennert, O. M.

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AB - The key enzymes in the synthesis of the naturally occurring polyamines, ornithine decarboxylase (ODC) and S-adenosyl methionine (SAM) decarboxylase, were investigated during cell growth and aging in fibroblast cultures from normal patients and patients with cystic fibrosis. A linear correlation between increased S-adenosyl methionine ine activity and putrescine concentration was apparent in all cell lines. A putrescine concentration of 0.8 mM was optimal for enhancement of SAM decarboxylase activity. The passage number of the cell line correlated inversely with maximal putrescine stimulated SAM decarboxylase activity, earlier passage numbers having the highest specific activity (Fig. 1). No significant differences in basal or putrescine stimulated SAM decarboxylase activity increased as the cell lines approached confluence. Activity was lowest during exponential growth (Fig. 3). ODC activity was increased during early exponential growth and fell as cells reached confluence (Fig. 4). No differences in ODC activity and putrescine inhibition between the normal and cystic fibrosis cell cultures at equivalent points of exponential growth were noted.

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