Ornithine accumulation and metabolism in rat lens

Takashi Shiono, Peter F. Kador, Jin H. Kinoshita

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The non-protein amino acid ornithine is readily accumulated into rat lenses cultured in TC-199 bicarbonate medium. This accumulation, measured by the lens water/lens medium ratios of radiolabeled ornithine, appears to be the result of an apparent energy-dependent basic amino acid transport system. Moreover, competition studies suggest that increased levels of ornithine can depress the concomitant lenticular accumulation of arginine and lysine. Examination of the trichloroacetic acid protein precipitates of these cultured lenses indicates that the radiolabel from ornithine can also be incorporated into lens proteins as proline. This results from the conversion by ornithine aminotransferase (EC 2. 6. 1. 13) of radiolabeled ornithine to delta1-pyrroline-5-carboxylic acid (P5C), which is subsequently converted to proline by P5C reductase (EC 1. 5. 1. 2). This incorporation of radiolabel into cultured lens proteins is reduced upon addition of either 1 mm proline or P5C to the culture medium or inhibited by the addition of the protein synthesis inhibitor, puromycin.

Original languageEnglish (US)
Pages (from-to)421-429
Number of pages9
JournalExperimental Eye Research
Volume40
Issue number3
DOIs
StatePublished - Mar 1985

Fingerprint

Ornithine
Lenses
Proline
Crystallins
Carboxylic Acids
Basic Amino Acid Transport Systems
Ornithine-Oxo-Acid Transaminase
Puromycin
Trichloroacetic Acid
Protein Synthesis Inhibitors
Bicarbonates
Lysine
Culture Media
Arginine
Amino Acids
Water
pyrroline
Proteins

Keywords

  • amino acid transport
  • delta-pyrroline-5-carboxylate
  • gyrate atrophy
  • hyperornithinemia
  • metabolism
  • ornithine
  • proline
  • rat lens

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Ornithine accumulation and metabolism in rat lens. / Shiono, Takashi; Kador, Peter F.; Kinoshita, Jin H.

In: Experimental Eye Research, Vol. 40, No. 3, 03.1985, p. 421-429.

Research output: Contribution to journalArticle

Shiono, Takashi ; Kador, Peter F. ; Kinoshita, Jin H. / Ornithine accumulation and metabolism in rat lens. In: Experimental Eye Research. 1985 ; Vol. 40, No. 3. pp. 421-429.
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