Origin and evolution of the chloroplast trnK (matK) intron: A model for evolution of group II intron RNA structures

Georg Hausner, Robert Olson, Dawn Simon, Ian Johnson, Erin R. Sanders, Kenneth G. Karol, Richard M. McCourt, Steven Zimmerly

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The trnK intron of plants encodes the matK open reading frame (ORF), which has been used extensively as a phylogenetic marker for classification of plants. Here we examined the evolution of the trnK intron itself as a model for group II intron evolution in plants. Representative trnK intron sequences were compiled from species spanning algae to angiosperms, and four introns were newly sequenced. Phylogenetic analyses showed that the matK ORFs belong to the ML (mitochondrial-like) subclass of group II intron ORFs, indicating that they were derived from a mobile group II intron of the class. RNA structures of the introns were folded and analyzed, which revealed progressive RNA structural deviations and degenerations throughout plant evolution. The data support a model in which plant organellar group II introns were derived from bacterial-like introns that had "standard" RNA structures and were competent for self-splicing and mobility and that subsequently the ribozyme structures degenerated to ultimately become dependent upon host-splicing factors. We propose that the patterns of RNA structure evolution seen for the trnK intron will apply to the other group II introns in plants.

Original languageEnglish (US)
Pages (from-to)380-391
Number of pages12
JournalMolecular biology and evolution
Volume23
Issue number2
DOIs
StatePublished - Feb 1 2006

Fingerprint

Chloroplasts
chloroplast
Introns
RNA
introns
chloroplasts
Open Reading Frames
phylogenetics
open reading frames
algae
angiosperm
RNA primers
alga
Angiosperms
Catalytic RNA
phylogeny
Angiospermae
taxonomy

Keywords

  • Chloroplast
  • Group II intron
  • Maturase
  • matk
  • trnK

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Origin and evolution of the chloroplast trnK (matK) intron : A model for evolution of group II intron RNA structures. / Hausner, Georg; Olson, Robert; Simon, Dawn; Johnson, Ian; Sanders, Erin R.; Karol, Kenneth G.; McCourt, Richard M.; Zimmerly, Steven.

In: Molecular biology and evolution, Vol. 23, No. 2, 01.02.2006, p. 380-391.

Research output: Contribution to journalArticle

Hausner, Georg ; Olson, Robert ; Simon, Dawn ; Johnson, Ian ; Sanders, Erin R. ; Karol, Kenneth G. ; McCourt, Richard M. ; Zimmerly, Steven. / Origin and evolution of the chloroplast trnK (matK) intron : A model for evolution of group II intron RNA structures. In: Molecular biology and evolution. 2006 ; Vol. 23, No. 2. pp. 380-391.
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