Origin and conservation genetics of the threatened Ute ladies'-tresses, Spiranthes diluvialis (Orchidaceae)

Allen L. Szalanski, Gerry Steinauer, Richard Bischof, Jessica L Petersen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The Ute ladies'-tresses, Spiranthes diluvialis, is listed as a threatened orchid in west-central United States by the Federal government. Information on its origin and patterns of genetic variation is needed to develop effective conservation strategies for this species. DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to evaluate genetic variation and structure of 23 populations of S. diluvialis. In addition, four congeneric species were analyzed to determine possible origins of the putative allotetraploid S. diluvialis. DNA sequencing and PCR-RFLP analysis of the nuclear ribosomal internal transcribed spacer (ITS) and mitochondrial and chloroplast DNA noncoding regions revealed no genetic variation within or among populations of S. diluvialis. DNA sequencing revealed that S. diluvialis has rDNA of both S. magnicamporum and S. romanzoffiana, supporting the proposed origin of the allotetraploid. Parsimony and maximum likelihood analyses of cpDNA and mtDNA sequences revealed that these S. diluvialis organellar sequences were most closely related to those of S. romanzoffiana, providing evidence that the latter species is the maternal parent of S. diluvialis. The lack of genetic diversity is significant for the development of a long-term conservation strategy for S. diluvialis.

Original languageEnglish (US)
Pages (from-to)177-180
Number of pages4
JournalAmerican Journal of Botany
Volume88
Issue number1
DOIs
StatePublished - Jan 1 2001

Fingerprint

Spiranthes
Orchidaceae
conservation genetics
germplasm conservation
DNA Sequence Analysis
genetic variation
DNA
sequence analysis
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase
chloroplast DNA
Mitochondrial DNA
Restriction Fragment Length Polymorphisms
polymerase chain reaction
restriction fragment length polymorphism
polymorphism
mitochondrial DNA
Chloroplast DNA
State Government
federal government

Keywords

  • Orchidaceae
  • Phylogenetics
  • Population genetics
  • Spiranthes
  • Threatened species

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Cite this

Origin and conservation genetics of the threatened Ute ladies'-tresses, Spiranthes diluvialis (Orchidaceae). / Szalanski, Allen L.; Steinauer, Gerry; Bischof, Richard; Petersen, Jessica L.

In: American Journal of Botany, Vol. 88, No. 1, 01.01.2001, p. 177-180.

Research output: Contribution to journalArticle

Szalanski, Allen L. ; Steinauer, Gerry ; Bischof, Richard ; Petersen, Jessica L. / Origin and conservation genetics of the threatened Ute ladies'-tresses, Spiranthes diluvialis (Orchidaceae). In: American Journal of Botany. 2001 ; Vol. 88, No. 1. pp. 177-180.
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