Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage

Marketa Kaucka, Tomas Zikmund, Marketa Tesarova, Daniel Gyllborg, Andreas Hellander, Josef Jaros, Jozef Kaiser, Julian Petersen, Bara Szarowska, Phillip T. Newton, Vyacheslav Dyachuk, Lei Li, Hong Qian, Anne Sofie Johansson, Yuji Mishina, Joshua D. Currie, Elly M. Tanaka, Alek Erickson, Andrew T Dudley, Hjalmar Brismar & 9 others Paul Southam, Enrico Coen, Min Chen, Lee S. Weinstein, Ales Hampl, Ernest Arenas, Andrei S. Chagin, Kaj Fried, Igor Adameyko

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cartilaginous structures are at the core of embryo growth and shaping before the bone forms. Here we report a novel principle of vertebrate cartilage growth that is based on introducing transversally-oriented clones into pre-existing cartilage. This mechanism of growth uncouples the lateral expansion of curved cartilaginous sheets from the control of cartilage thickness, a process which might be the evolutionary mechanism underlying adaptations of facial shape. In rod-shaped cartilage structures (Meckel, ribs and skeletal elements in developing limbs), the transverse integration of clonal columns determines the well-defined diameter and resulting rod-like morphology. We were able to alter cartilage shape by experimentally manipulating clonal geometries. Using in silico modeling, we discovered that anisotropic proliferation might explain cartilage bending and groove formation at the macro-scale.

Original languageEnglish (US)
Article numbere25902
JournaleLife
Volume6
DOIs
StatePublished - Apr 17 2017

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Cartilage
Vertebrates
Growth
Ribs
Computer Simulation
Macros
Bone
Embryonic Structures
Extremities
Clone Cells
Bone and Bones
Geometry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Kaucka, M., Zikmund, T., Tesarova, M., Gyllborg, D., Hellander, A., Jaros, J., ... Adameyko, I. (2017). Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. eLife, 6, [e25902]. https://doi.org/10.7554/eLife.25902

Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. / Kaucka, Marketa; Zikmund, Tomas; Tesarova, Marketa; Gyllborg, Daniel; Hellander, Andreas; Jaros, Josef; Kaiser, Jozef; Petersen, Julian; Szarowska, Bara; Newton, Phillip T.; Dyachuk, Vyacheslav; Li, Lei; Qian, Hong; Johansson, Anne Sofie; Mishina, Yuji; Currie, Joshua D.; Tanaka, Elly M.; Erickson, Alek; Dudley, Andrew T; Brismar, Hjalmar; Southam, Paul; Coen, Enrico; Chen, Min; Weinstein, Lee S.; Hampl, Ales; Arenas, Ernest; Chagin, Andrei S.; Fried, Kaj; Adameyko, Igor.

In: eLife, Vol. 6, e25902, 17.04.2017.

Research output: Contribution to journalArticle

Kaucka, M, Zikmund, T, Tesarova, M, Gyllborg, D, Hellander, A, Jaros, J, Kaiser, J, Petersen, J, Szarowska, B, Newton, PT, Dyachuk, V, Li, L, Qian, H, Johansson, AS, Mishina, Y, Currie, JD, Tanaka, EM, Erickson, A, Dudley, AT, Brismar, H, Southam, P, Coen, E, Chen, M, Weinstein, LS, Hampl, A, Arenas, E, Chagin, AS, Fried, K & Adameyko, I 2017, 'Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage', eLife, vol. 6, e25902. https://doi.org/10.7554/eLife.25902
Kaucka M, Zikmund T, Tesarova M, Gyllborg D, Hellander A, Jaros J et al. Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. eLife. 2017 Apr 17;6. e25902. https://doi.org/10.7554/eLife.25902
Kaucka, Marketa ; Zikmund, Tomas ; Tesarova, Marketa ; Gyllborg, Daniel ; Hellander, Andreas ; Jaros, Josef ; Kaiser, Jozef ; Petersen, Julian ; Szarowska, Bara ; Newton, Phillip T. ; Dyachuk, Vyacheslav ; Li, Lei ; Qian, Hong ; Johansson, Anne Sofie ; Mishina, Yuji ; Currie, Joshua D. ; Tanaka, Elly M. ; Erickson, Alek ; Dudley, Andrew T ; Brismar, Hjalmar ; Southam, Paul ; Coen, Enrico ; Chen, Min ; Weinstein, Lee S. ; Hampl, Ales ; Arenas, Ernest ; Chagin, Andrei S. ; Fried, Kaj ; Adameyko, Igor. / Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. In: eLife. 2017 ; Vol. 6.
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