Organ site-dependent expression of basic fibroblast growth factor in human renal cell carcinoma cells

Rakesh K Singh, Corazon D. Bucana, Mordechai Gutman, Dominic Fan, Michael R. Wilson, Isaiah J. Fidler

Research output: Contribution to journalArticle

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Abstract

We investigated the influence of organ microenvironment on the angiogenic phenotype in human renal cell carcinoma (HRCC) cells. HRCC line SN12G was established in vitro from a surgical specimen, and metastatic line SN12PM6 was isolated from a lung metastasis produced by parental cells implanted into the kidney of nude mice. SN12G (low metastasis) and SN12PM6 (high metastasis) cells were injected into the kidney or subculis of nude mice. The kidney tumors were highly vascularized (as revealed by immunohistochemistry using antibodies against factor VIII), and metastatic, whereas the subcutaneous tumors were not. The expression of mRNA for basic fibroblast growth factor (bFGF) in kidney tumors was 10 to 20 times that found in subcutaneous tumors. Similar data were obtained at the protein level by using fluorescence activated cell sorting, immunohistochemistry, and enzyme-linked immunosorbent assay, bFGF was detected in the urine of mice with tumors in the kidney but not subcutaneous tumors. The level of bFGF in the serum of mice with kidney tumors was two to three times that in mice with subcutaneous tumors. The changes in bFGF expression in the tumors was transient. Collectively; these data indicate that the organ microenvironment can influence the expression level of bFGF in HRCC.

Original languageEnglish (US)
Pages (from-to)365-374
Number of pages10
JournalAmerican Journal of Pathology
Volume145
Issue number2
StatePublished - Aug 1 1994

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Fibroblast Growth Factor 2
Renal Cell Carcinoma
Kidney
Neoplasms
Neoplasm Metastasis
Nude Mice
Immunohistochemistry
Factor VIII
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Urine
Phenotype
Cell Line
Lung
Messenger RNA
Antibodies
Serum

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Singh, R. K., Bucana, C. D., Gutman, M., Fan, D., Wilson, M. R., & Fidler, I. J. (1994). Organ site-dependent expression of basic fibroblast growth factor in human renal cell carcinoma cells. American Journal of Pathology, 145(2), 365-374.

Organ site-dependent expression of basic fibroblast growth factor in human renal cell carcinoma cells. / Singh, Rakesh K; Bucana, Corazon D.; Gutman, Mordechai; Fan, Dominic; Wilson, Michael R.; Fidler, Isaiah J.

In: American Journal of Pathology, Vol. 145, No. 2, 01.08.1994, p. 365-374.

Research output: Contribution to journalArticle

Singh, RK, Bucana, CD, Gutman, M, Fan, D, Wilson, MR & Fidler, IJ 1994, 'Organ site-dependent expression of basic fibroblast growth factor in human renal cell carcinoma cells', American Journal of Pathology, vol. 145, no. 2, pp. 365-374.
Singh, Rakesh K ; Bucana, Corazon D. ; Gutman, Mordechai ; Fan, Dominic ; Wilson, Michael R. ; Fidler, Isaiah J. / Organ site-dependent expression of basic fibroblast growth factor in human renal cell carcinoma cells. In: American Journal of Pathology. 1994 ; Vol. 145, No. 2. pp. 365-374.
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