Oral administration of lens homogenate suppresses production of lens antibodies in mice immunized with lens antigens

T. Sueno, D. P. Singh, L. T. Chylack, T. Shinohara

Research output: Contribution to journalArticle

Abstract

Purpose: Circulating autoantibodies (Ab) against lens antigens (Ag) in patients with cataract, when presented with complement, kill lens epithelial cells (LECs) in vitro. To suppress production of cytotoxic Abs, we initiated a study of the effect of oral administration of lens Ags on this immune response. Methods: Lens Ags were administered orally before and after immunization with lens Ag. Young mice (1.5 mos) were fed calf lens homogenate, 4mg/mouse, every 4 days for 5 weeks, before and after immunization with β-crystallins emulsified with CFA. Sera from these animals were analyzed by ELISA and protein blot analysis against β-crystallins. Results: The titer of Ab gradually increased to 2X106 by the 5th week in the control animals. In contrast, in animals fed lens-Ag, the levels of Ab were reduced by 40-60% compared to control animals. Levels of Ab were more strongly suppressed in animals fed before immunization than after immunization. Abs against β-crystallins were suppressed by this treatment. In addition, the proliferative response of T-lymphocytes against lens-Ag was suppressed. This suppression appears to inhibit the CD4-mediated interaction either between T-cell receptor and MHC molecules on the Ag presenting cells or secretion of immune suppressive factors by T-cells.. Conclusions: Thus, oral administration of antigenic protein is a specific and non-toxic method to suppress a specific immune response to the same Ag. We are exploring the therapeutic value of oral administration of lens proteins in age-related cataract.

Original languageEnglish (US)
Pages (from-to)S754
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

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Lenses
Antibody Formation
Oral Administration
Antigens
Crystallins
Autoantibodies
Immunization
Cataract
T-Lymphocytes
Immunologic Factors
Antigen-Presenting Cells
T-Cell Antigen Receptor
Proteins
Epithelial Cells
Enzyme-Linked Immunosorbent Assay
Therapeutics
Serum

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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Oral administration of lens homogenate suppresses production of lens antibodies in mice immunized with lens antigens. / Sueno, T.; Singh, D. P.; Chylack, L. T.; Shinohara, T.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996, p. S754.

Research output: Contribution to journalArticle

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abstract = "Purpose: Circulating autoantibodies (Ab) against lens antigens (Ag) in patients with cataract, when presented with complement, kill lens epithelial cells (LECs) in vitro. To suppress production of cytotoxic Abs, we initiated a study of the effect of oral administration of lens Ags on this immune response. Methods: Lens Ags were administered orally before and after immunization with lens Ag. Young mice (1.5 mos) were fed calf lens homogenate, 4mg/mouse, every 4 days for 5 weeks, before and after immunization with β-crystallins emulsified with CFA. Sera from these animals were analyzed by ELISA and protein blot analysis against β-crystallins. Results: The titer of Ab gradually increased to 2X106 by the 5th week in the control animals. In contrast, in animals fed lens-Ag, the levels of Ab were reduced by 40-60{\%} compared to control animals. Levels of Ab were more strongly suppressed in animals fed before immunization than after immunization. Abs against β-crystallins were suppressed by this treatment. In addition, the proliferative response of T-lymphocytes against lens-Ag was suppressed. This suppression appears to inhibit the CD4-mediated interaction either between T-cell receptor and MHC molecules on the Ag presenting cells or secretion of immune suppressive factors by T-cells.. Conclusions: Thus, oral administration of antigenic protein is a specific and non-toxic method to suppress a specific immune response to the same Ag. We are exploring the therapeutic value of oral administration of lens proteins in age-related cataract.",
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